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Functional characterization of the soybean cyst nematode effector SCN-27D09 using the model plant pathogenic fungus Magnaporthe oryzae-mediated delivery system
Phytopathology Research Pub Date : 2022-09-13 , DOI: 10.1186/s42483-022-00138-w
Nan Yang , Qianqian Yu , Wenhao Li , Deng Chen , Jinzhuo Jian , Huixia Zhang , Gaofeng Wang , Shiming Liu , Wenkun Huang , Huan Peng , Deliang Peng , Jun Yang , Xiaoli Guo , Lingan Kong

Soybean cyst nematode (SCN, Heterodera glycines) is widely considered as the model plant-parasitic nematode, which secretes effector proteins to manipulate host responses. In this study, we cloned a dorsal gland-expressed effector protein SCN-27D09 that belongs to the same family as Hg10A07 in SCN. We used the model plant pathogen rice blast fungus (Magnaporthe oryzae) to quickly predict and characterize the functions of SCN-27D09. By using M. oryzae secretion system in barley, we confirmed that the signal peptide of SCN-27D09 has secretory activity and can guide the protein into the host cells. Heterologous expression of SCN-27D09 in M. oryzae significantly enhanced the susceptibility of barley to M. oryzae. SCN-27D09 can inhibit Bax-triggered cell death when expressed in Nicotiana benthamiana. Overexpression of SCN-27D09 in soybean hairy root also increased the susceptibility of soybean plants to SCN. Moreover, yeast two-hybrid and firefly luciferase complementation imaging assays showed that SCN-27D09 interacts with a soybean plant kinase GmIPK-2. Functional characterization of GmIPK-2 revealed its positive role in soybean resistance, indicating that SCN-27D09 might compromise the function of GmIPK-2 to facilitate nematode infection. Our results not only uncover the biological role of SCN-27D09 in suppressing plant defense responses and therefore promoting nematode parasitism, but also reaffirm the potential application of the model plant pathogenic fungus M. oryzae in investigating the pathogenic roles of candidate effectors of phytonematodes.



中文翻译:

使用模式植物病原真菌稻瘟病菌介导的递送系统对大豆胞囊线虫效应器 SCN-27D09 的功能表征

大豆胞囊线虫(SCN,Heterodera glycines)被广泛认为是植物寄生线虫的模型,它分泌效应蛋白来操纵宿主反应。在这项研究中,我们克隆了背腺表达的效应蛋白 SCN-27D09,它与 SCN 中的 Hg10A07 属于同一家族。我们使用模式植物病原体稻瘟病菌 ( Magnaporthe oryzae ) 快速预测和表征 SCN-27D09 的功能。通过使用大麦中的M. oryzae分泌系统,我们证实SCN-27D09的信号肽具有分泌活性,可以引导蛋白质进入宿主细胞。SCN-27D09M. oryzae中的异源表达显着增强了大麦对M. oryzaeSCN-27D09 在本氏烟草中表达时可抑制 Bax 引发的细胞死亡。SCN-27D09在大豆毛状根中的过表达也增加了大豆植物对SCN的敏感性。此外,酵母双杂交和萤火虫荧光素酶互补成像分析表明,SCN-27D09 与大豆植物激酶 GmIPK-2 相互作用。GmIPK-2的功能表征揭示其在大豆抗性中的积极作用,表明 SCN-27D09 可能会损害 GmIPK-2 的功能以促进线虫感染。我们的研究结果不仅揭示了 SCN-27D09 在抑制植物防御反应并因此促进线虫寄生中的生物学作用,而且再次证实了模式植物病原真菌M. oryzae在研究植物线虫候选效应物的致病作用方面的潜在应用。

更新日期:2022-09-13
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