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Simultaneous Determination of the Saponification Value, Acid Value, Ester Value, and Iodine Value in Commercially Available Red Fruit Oil (Pandanus conoideus, Lam.) Using 1H qNMR Spectroscopy
Food Analytical Methods ( IF 2.6 ) Pub Date : 2022-09-13 , DOI: 10.1007/s12161-022-02401-4
Liling Triyasmono , Curd Schollmayer , Jens Schmitz , Emilie Hovah , Cristian Lombo , Sebastian Schmidt , Ulrike Holzgrabe

Red fruit oil (RFO) can be extracted from fruits of Pandanus conoideus, Lam., an endogenous plant of Papua, Indonesia. It is a commonly used essential original traditional medicine. By applying a newly developed quantitative 1H NMR (qNMR) spectroscopy method for quality assessment, a simultaneous determination of the saponification value (SV), acid value (AV), ester value (EV), and iodine value (IV) in RFO was possible. Dimethyl sulfone (DMSO2) was used as an internal standard. Optimization of NMR parameters, such as NMR pulse sequence, relaxation delay time, and receiver gain, finally established the 1H NMR-based quantification approach. Diagnostic signals of the internal standard at δ = 2.98 ppm, SV at δ = 2.37–2.20 ppm, AV at δ = 2.27–2.20 ppm, EV at δ = 2.37–2.27 ppm, and IV at δ = 5.37–5.27 ppm, respectively, were used for quantitative analysis. The method was validated concerning linearity (R2 = 0.999), precision (less than 0.83%), and repeatability in the range 99.17–101.17%. Furthermore, this method was successfully applied to crude RFO, crude RFO with palmitic and oleic acid addition, and nine commercial products. The qNMR results for the respective fat values are in accordance with the results of standard methods, as can be seen from the F- and t-test (< 1.65 and < 1.66, respectively). The fundamental advantages of qNMR, such as its rapidity and simplicity, make it a feasible and existing alternative to titration for the quality control of RFO.



中文翻译:

使用 1H qNMR 光谱法同时测定市售红果油 (Pandanus conoideus, Lam.) 中的皂化值、酸值、酯值和碘值

红色果油 (RFO) 可从印度尼西亚巴布亚的一种内源植物Pandanus conoideus, Lam. 的果实中提取。是一种常用的必需原药。通过应用新开发的定量1 H NMR (qNMR) 光谱方法进行质量评估,同时测定 RFO 中的皂化值 (SV)、酸值 (AV)、酯值 (EV) 和碘值 (IV)可能的。二甲基砜(DMSO 2)用作内标。对核磁共振脉冲序列、弛豫延迟时间、接收器增益等核磁参数进行优化,最终确定了1基于 H NMR 的量化方法。δ = 2.98 ppm 时的内标诊断信号、δ = 2.37–2.20 ppm 时的 SV、δ = 2.27–2.20 ppm 时的 AV、δ = 2.37–2.27 ppm 时的 EV 和 δ = 5.37–5.27 ppm 时的 IV 的诊断信号,分别, 用于定量分析。该方法在线性度(R 2  = 0.999)、精密度(小于 0.83%)和 99.17-101.17% 范围内的重复性方面进行了验证。此外,该方法成功地应用于粗RFO、添加棕榈酸和油酸的粗RFO以及九种商业产品。各脂肪值的 qNMR 结果与标准方法的结果一致,从F - 和t可以看出-测试(分别 < 1.65 和 < 1.66)。qNMR 的基本优势,如快速和简单,使其成为 RFO 质量控制滴定的可行和现有替代方案。

更新日期:2022-09-14
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