Nanoconfinement – amplified, Förster resonance energy transfer in nile red – borne mesoporous silica for ultrasensitive, multiplex assay of triphenylmethane dyes in aqueous milieu,Materials Chemistry Frontiers

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Nanoconfinement – amplified, Förster resonance energy transfer in nile red – borne mesoporous silica for ultrasensitive, multiplex assay of triphenylmethane dyes in aqueous milieu
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2022-09-13 , DOI: 10.1039/d2qm00709f
Yifan Chen ₁ , Sijian Ye ₁ , Chongling Cheng ₁ , Renguo Xie ₁ , Dayang Wang ₁

Affiliation

In this work, lipophilic Nile red (NR) was loaded into the mesopores of MCM-41 particles via hydrogen bonding-driven adsorption in organic media, and the resulting NR-borne MCM-41 particles were harnessed as mesoporous luminescent donors to detect hydrophilic triphenylmethane (TPM) dyes in aqueous media based on NR-to-TPM Förster resonance energy transfer (FRET). This new approach enabled us to determine the TPM concentration ranging from ca. 1 μmol L⁻¹ to ca. 1 nmol L⁻¹ with the detection limit at the level of 0.1 nmol L⁻¹ (0.1 ppb) as a result of about 1000-fold enrichment of the TPM dyes in the NR-borne MCM-41 mesopores thanks to nanoconfinement. The TPM-induced fluorescence quenching constant of NR-borne MCM-41 particles, obtained according to the Stern–Volmer relationship, was empirically connected with their NR uptake in a log-linear manner, the slope of which enabled one to identify the molecular nature of TPM dyes. Furthermore, a rough-and-read protocol of image analysis and processing was developed to convert the fluorescence images of microtiter plates with wells filled with NR-borne MCM-41 particles and aqueous TPM solutions into the multiplex, FRET-encoded matrices, which enabled us to simultaneously realize molecular identification of the targeted TPM analytes and sub-ppb-level determination of their concentrations.

中文翻译：

尼罗红介孔二氧化硅中的纳米限制 - 放大的 Förster 共振能量转移，用于水环境中三苯甲烷染料的超灵敏、多重分析

在这项工作中，亲脂性尼罗红（NR）通过有机介质中的氢键驱动吸附加载到 MCM-41 颗粒的介孔中，并利用所得 NR 载 MCM-41 颗粒作为介孔发光供体来检测亲水性三苯甲烷(TPM) 基于 NR 到 TPM Förster 共振能量转移 (FRET) 的水性介质中的染料。这种新方法使我们能够确定 TPM 浓度范围为ca。1 μmol L ^-1至约。1 nmol L ^-1检测限为 0.1 nmol L ^-1（0.1 ppb），由于纳米限制使 NR 承载的 MCM-41 中孔中的 TPM 染料富集约 1000 倍。根据 Stern-Volmer 关系获得的 NR 载 MCM-41 颗粒的 TPM 诱导的荧光猝灭常数与它们的 NR 摄取以对数线性方式凭经验相关，其斜率使人们能够识别分子性质TPM 染料。此外，还开发了一种图像分析和处理的粗略读取协议，以将装有 NR 载 MCM-41 颗粒和 TPM 水溶液的孔的微量滴定板的荧光图像转换为多重 FRET 编码矩阵，这使得我们同时实现了目标TPM分析物的分子鉴定和亚ppb水平的浓度测定。

更新日期：2022-09-13

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