Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus,SYNAPSE

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Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus
SYNAPSE ( IF 1.6 ) Pub Date : 2022-09-09 , DOI: 10.1002/syn.22250
Miriam E Reyes-Méndez ₁ , J Manuel Herrera-Zamora ₁ , Fernando Osuna-Lopez ₁ , Irving S Aguilar-Martínez ₁ , José L Góngora-Alfaro ₂ , Ricardo A Navarro-Polanco ₁ , Enrique Sánchez-Pastor ₁ , Eloy G Moreno-Galindo ₁ , Javier Alamilla _{1,

3}

Affiliation

The suprachiasmatic nucleus (SCN) is the most important circadian clock in mammals. The SCN synchronizes to environmental light via the retinohypothalamic tract (RHT), which is an axon cluster derived from melanopsin-expressing intrinsic photosensitive retinal ganglion cells. Investigations on the development of the nonimage-forming pathway and the RHT are scarce. Previous studies imply that light stimulation during postnatal development is not needed to make the RHT functional at adult stages. Here, we examined the effects of light deprivation (i.e., constant darkness (DD) rearing) during postnatal development on the expression in the ventral SCN of two crucial proteins for the synchronization of circadian rhythms to light: the presynaptic vesicular glutamate transporter type 2 (vGluT2) and the GluN2B subunit of the postsynaptic NMDA receptor. We found that animals submitted to DD conditions exhibited a transitory reduction in the expression of vGluT2 (at P12–19) and of GluN2B (at P7–9) that was compensated at older stages. These findings support the hypothesis that visual stimulation during early ages is not decisive for normal development of the RHT-SCN pathway.

中文翻译：

发育性光剥夺瞬时降低大鼠腹侧视交叉上核 vGluT2 和 GluN2B 的表达

视交叉上核 (SCN) 是哺乳动物最重要的生物钟。SCN 通过视网膜下丘脑束 (RHT) 与环境光同步，视网膜下丘脑束是源自表达黑视素的内在感光性视网膜神经节细胞的轴突簇。关于非图像形成途径和 RHT 发展的研究很少。以前的研究表明，不需要出生后发育过程中的光刺激来使 RHT 在成年阶段发挥作用。在这里，我们检查了出生后发育过程中光照剥夺（即持续黑暗 (DD) 饲养）对腹侧 SCN 中两种重要蛋白质表达的影响，这些蛋白质用于使昼夜节律与光照同步：2 型突触前囊泡谷氨酸转运蛋白（ vGluT2) 和突触后 NMDA 受体的 GluN2B 亚基。我们发现提交给 DD 条件的动物表现出 vGluT2（在 P12-19）和 GluN2B（在 P7-9）的表达暂时减少，这在较早的阶段得到补偿。这些发现支持这样的假设，即早期的视觉刺激对于 RHT-SCN 通路的正常发育不是决定性的。

更新日期：2022-09-09

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