聚苯乙烯颗粒概念的介电泳证明和体外人表皮角质形成细胞迁移用于伤口再生,Journal of Applied Polymer Science

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聚苯乙烯颗粒概念的介电泳证明和体外人表皮角质形成细胞迁移用于伤口再生
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2022-09-10 , DOI: 10.1002/app.53096
Revathy Deivasigamani ₁ , Nur Nasyifa Mohd Maidin ₁ , Nur Shahira Abdul Nasir ₁ , Mei Xian Low _{2,

3} , Aminuddin Bin Ahmad Kayani _{2,

3} , Mohd Ambri Mohamed ₁ , Muhamad Ramdzan Buyong ₁

Affiliation

糖尿病影响全球约 1.7 亿人，预计到 2030 年将增加一倍，是一个严重的问题。通过介电泳 (DEP) 技术进行的电刺激 (ES) 可能是提高开放性溃疡糖尿病患者愈合率的有效替代方法。本研究使用 DEP 力 (F _DEP) 操纵 3.2、4.8、10 和 15 μm 聚苯乙烯 (PS) 颗粒来预测人表皮角质形成细胞 (HEK) 的迁移能力。数值建模方法 MyDEP 用于预测 PS 粒子和 HEK 的克劳修斯-莫索蒂因子的解释。有限元方法基于 DEP 和各自介质中的阻力计算电场强度和粒子轨迹。使用非均匀电场在锥形铝微电极阵列中对多种尺寸的 PS 颗粒和活 HEK 进行 DEP 实验。不同的 PS 粒子表现出正 DEP (P _DEP )、交叉频率 (f _XO ) 和负 DEP (N _DEP )，而 HEK 的经验只有 N _DEP由于其在频率范围为 100 kHz 至 1 MHz 的高导电介质。最后，DIPP-MotionV 分析表明，速度和加速度之间的粒子迁移率在统计上相当可观。当适当的频率应用于随机位置的 HEK 时，F _DEP会根据其介电特性在所需的目标位置对齐，从而加速体内条件下的伤口愈合。

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A dielectrophoresis proof of concept of polystyrene particles and in-vitro human epidermal keratinocytes migration for wound rejuvenation

Diabetes affects approximately 170 million people worldwide, is expected to double by 2030, and is a severe problem. Electrical stimulation (ES) via dielectrophoresis (DEP) technique may be an effective alternative in enhancing healing rates in diabetic patients with open ulcers. This research used DEP force (F_DEP) to manipulate 3.2, 4.8, 10, and 15 μm polystyrene (PS) particles to predict the migration capability of human epidermal keratinocytes (HEKs). A numerical modeling method, MyDEP, was used to predict the interpretation of Clausius–Mossotti factors of PS particles and HEKs. The finite element method computes the electric field intensity and particle trajectory based on DEP and drag forces in their respective medium. DEP experiments on numerous size PS particles and alive HEKs were carried out in a tapered aluminium microelectrode array using a non-uniform electric field. The distinct PS particles exhibit positive DEP (P_DEP), crossover frequency (f_XO), and negative DEP (N_DEP), whereas the HEKs experience, only N_DEP due to its high conductive medium in frequency ranges from 100 kHz to 1 MHz. Finally, the DIPP-MotionV analysis shows that particle mobility between speed and acceleration is statistically considerable. When an appropriate frequency is applied to HEKs in random locations, the F_DEP aligns at the desired target position based on its dielectric properties, which accelerates wound healing in in-vivo conditions.

更新日期：2022-09-10

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