An Atypical Acyl-CoA Synthetase Enables Efficient Biosynthesis of Extender Units for Engineering a Polyketide Carbon Scaffold,Angewandte Chemie International Edition

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An Atypical Acyl-CoA Synthetase Enables Efficient Biosynthesis of Extender Units for Engineering a Polyketide Carbon Scaffold
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2022-09-08 , DOI: 10.1002/anie.202208734
Mengmeng Zheng _{1,

2} , Jun Zhang ₂ , Wan Zhang ₁ , Lu Yang ₂ , Xiaoli Yan ₁ , Wenya Tian ₂ , Zhihao Liu ₁ , Zhi Lin ₂ , Zixin Deng ₂ , Xudong Qu _{1,

2}

Affiliation

A new acyl-CoA synthetase (ACS, UkaQ) with broad substrate specificity and an unusual catalytic mode was identified. Its stability and catalytic activity were remarkably improved by protein engineering, enabling it to synthesize a large variety of acyl-CoAs. In combination with permissive carboxylases, diverse extender units were synthesized and used to engineer the polyketide carbon scaffold of antimycin.

中文翻译：

一种非典型的酰基辅酶 A 合成酶能够有效地生物合成扩展单元，用于设计聚酮化合物碳支架

鉴定了一种具有广泛底物特异性和不寻常催化模式的新型酰基辅酶 A 合成酶（ACS，UkaQ）。蛋白质工程显着提高了其稳定性和催化活性，使其能够合成多种酰基辅酶 A。结合允许的羧化酶，合成了多种扩展单元，并用于设计抗霉素的聚酮化合物碳支架。

更新日期：2022-09-08

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