ABSTRACT

Aflatoxins are the most potent hepatocarcinogens of all biologically produced toxins found to date. Contaminated food and commodities are one of the most serious problems due to an irreversible damage to health. Taking advantage of biological methods to improve food safety, an aflatoxin B₁ (AFB₁) bioremoval study was conducted to investigate the potential of arrested fungal cells on magnetic nanoparticles. The favourable results on the degradation of AFB₁ indicate a reasonable influence of the carriers on the fungal cells and the degradation process. The efficacy of degradation of AFB₁ by free and immobilised fungal cells on four different magnetic nanoparticles (Fe₃O₄) was investigated. The degradation rate by HPLC analysis exhibited 54.97% for the free cell study, 85.85% for Fe₃O₄@MTMS@cell, 95.07% for Fe₃O₄@TEOS@cell, 84.48% for Fe₃O₄@SiO₂-NH₂@cell and 96.52% for Fe₃O₄@BCD@cell for the 3 μg/ml AFB₁. The degradation with Fe₃O₄@ BCD@cell showed remarkable results even for 5 μg/ml AFB₁ resulting 93.42%.

摘要

黄曲霉毒素是迄今为止发现的所有生物产生的毒素中最有效的肝癌物质。由于对健康造成不可逆转的损害，受污染的食品和商品是最严重的问题之一。利用生物方法来提高食品安全性，进行了一项黄曲霉毒素 B ₁ (AFB ₁ ) 生物去除研究，以研究在磁性纳米粒子上捕获的真菌细胞的潜力。_{AFB 1}降解的有利结果表明载体对真菌细胞和降解过程的影响是合理的。_{四种不同磁性纳米颗粒（Fe 3} O ₄）上游离和固定化真菌细胞降解 AFB _1的效果) 进行了调查。通过 HPLC 分析显示，游离细胞研究的降解率为 54.97%，Fe ₃ O ₄ @MTMS@cell为 85.85%，Fe ₃ O ₄ @TEOS@cell 为 95.07%，Fe ₃ O ₄ @SiO ₂为 84.48% - NH ₂ @cell 和 96.52% 对于 Fe ₃ O ₄ @BCD@cell 对于 3 μg/ml AFB ₁。_{即使对于 5 μg/ml AFB 1}，Fe ₃ O ₄ @BCD@cell 的降解也显示出显着的结果，达到93.42%。