柠檬酸配位的羟基磷灰石纳米颗粒膜对蛋白质吸附和细胞粘附状态的纳米空间影响,Journal of Materials Chemistry B

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柠檬酸配位的羟基磷灰石纳米颗粒膜对蛋白质吸附和细胞粘附状态的纳米空间影响
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2022-09-05 , DOI: 10.1039/d2tb01240e
Zizhen Liu ₁ , Daisuke Kawagoe ₂ , Motohiro Tagaya ₁

Affiliation

使用具有不同纳米空间的羟基磷灰石（HA）和柠檬酸（Cit）配位的 HA（Cit / HA）纳米颗粒膜，通过稳定性和稳定性的前提来检查纳米空间对蛋白质吸附行为和初始成骨细胞样细胞粘附能力的影响。薄膜在生物溶液中的离子解离特性。特别是，与 HA 纳米颗粒膜相比，纳米空间为 4.2 nm 的 Cit/HA 纳米颗粒膜由于其更大的比表面积和更小的纳米空间，可以实现蛋白质的大量立体吸附。还发现Cit/HA纳米粒子薄膜上吸附的胎牛血清蛋白的α-螺旋和（β-折叠+β-转角）组分比通过二级结构与HA纳米粒子薄膜相比有所增加分析吸附的蛋白质，这有助于薄膜表面良好的初始细胞培养特性。因此，我们成功地利用不同的纳米空间 HA 和 Cit/HA 纳米颗粒薄膜实现了对蛋白质吸附状态的控制，从而获得了优异的初始细胞培养特性，这将为再生医学领域中新型细胞培养基质表面的创造提供新的见解。

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Nanospacial effect of citric acid-coordinated hydroxyapatite nanoparticle films on protein adsorption and cell adhesion states

Hydroxyapatite (HA) and citric acid (Cit)-coordinated HA (Cit/HA) nanoparticle films with different nanospaces were used to examine the nanospacial effect on the protein adsorption behavior and initial osteoblast-like cell adhesion ability through the premise of the stability and ionic dissociation characteristics of the films in biological solution. In particular, the Cit/HA nanoparticle film with a nanospace of 4.2 nm could realize massive and stereoscopic adsorption of proteins due to its larger specific surface area and smaller nanospace as compared with the case of the HA nanoparticle film. It was also found that the α-helix and (β-sheet + β-turn) component ratios of the adsorbed fetal bovine serum proteins on the Cit/HA nanoparticle films increased as compared with the case of the HA nanoparticle film through the secondary structure analysis of the adsorbed proteins, which contributed to the good initial cell culture properties on the film surfaces. Therefore, we successfully realized the control of protein adsorption states using different nanospacial HA and Cit/HA nanoparticle films to achieve excellent initial cell culture properties, which would provide new insights into the creation of novel cell culture substrate surfaces in the regenerative medicine fields.

更新日期：2022-09-05

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