Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2022-09-02 , DOI: 10.1016/j.ibmb.2022.103831
Katherine Barie 1 , Eran Levin 2 , Etya Amsalem 1
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Carbon dioxide (CO2) has pleiotropic effects on insect physiology and behavior. Although diverse, many impacts are related to changes in metabolism and reallocation of macronutrients. Here we examined the metabolic shift induced by CO2 and its regulation using Bombus impatiens. CO2 applied to bumble bee gynes induces bypass of diapause and transition into reproduction. We analyzed ovary activation and macronutrient amounts in four tissues/body parts (fat body, thorax, ovaries, and crop) at three timepoints following CO2 administration. To tease apart the effects of CO2 on reproduction and metabolism, we monitored the metabolic changes in gynes following ovary removal and CO2 narcosis. We also explored the role of juvenile hormone in mediating CO2 impact by feeding queens with a JH antagonist (Precocene). Gynes ovary activation was increased following CO2 treatment. Additionally, CO2-treated gynes showed lower lipid amount in the fat body and higher glycogen and protein amount in the ovary ten days after the treatment. CO2 treatment following ovary removal also resulted in decreased fat body lipids, suggesting that CO2 operates by inducing a metabolic shift independent of reproduction. Lastly, gynes fed with precocence did not show a metabolic shift following CO2, suggesting CO2 impact is mediated via juvenile hormone. Overall, these data suggest that CO2 induces transfer of macronutrients and utilization of stored reserved by accelerating metabolism. The proposed mechanism of CO2 may explain many of the pleiotropic effects of CO2 across species and can aid in understanding how this common anastatic influences insect physiology.
中文翻译:
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CO2 麻醉诱导通过保幼激素介导的 Bombus impatiens gynes 的代谢转变
二氧化碳(CO 2 )对昆虫生理和行为具有多效性。尽管多种多样,但许多影响与新陈代谢的变化和常量营养素的重新分配有关。在这里,我们检查了由 CO 2诱导的代谢转变及其使用凤仙花的调节。CO 2应用于大黄蜂gynes 诱导绕过滞育并过渡到繁殖。我们在施用CO 2后的三个时间点分析了四个组织/身体部位(脂肪体、胸部、卵巢和作物)中的卵巢活化和常量营养素含量。梳理 CO 2的影响在生殖和代谢方面,我们监测了卵巢切除和 CO 2麻醉后女性的代谢变化。我们还通过用 JH 拮抗剂 (Precocene) 喂养皇后来探索保幼激素在介导 CO 2影响中的作用。CO 2处理后女性卵巢活化增加。此外,CO 2处理的女性在处理十天后表现出脂肪体中的脂质含量较低,而卵巢中的糖原和蛋白质含量较高。卵巢切除后的CO 2处理也导致脂肪体脂质降低,这表明 CO 2通过诱导独立于繁殖的代谢转变来发挥作用。最后,用早产喂养的女性在 CO 2后没有表现出代谢变化,这表明 CO 2的影响是通过保幼激素介导的。总之,这些数据表明CO 2通过加速新陈代谢诱导大量营养素的转移和储存的利用。所提出的 CO 2机制可以解释 CO 2跨物种的许多多效性效应,并有助于理解这种常见的突变如何影响昆虫生理。