Integrated Acoustic Chip for Culturing 3D Cell Arrays,ACS Sensors

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Integrated Acoustic Chip for Culturing 3D Cell Arrays
ACS Sensors ( IF 8.2 ) Pub Date : 2022-09-01 , DOI: 10.1021/acssensors.2c01103
Yong Luo ₁ , Hongxiao Gao ₁ , Mengyun Zhou ₂ , Long Xiao ₃ , Tailin Xu _{1,

2} , Xueji Zhang ₂

Affiliation

Three-dimensional (3D) cell arrays provide an in vitro platform for clinical drug screening, but the bulky culture devices limit their application scenarios. Here, we demonstrate an integrated portable device that can realize contact-free construction of 3D cell spheroids. The interaction between the ultrasound generated by the portable device and the capillary results in periodic pressure nodes or anti-nodes, which lead to form a 3D cell array for cell culture. Such a 3D cell array pattern can be constructed in seconds and requires only 1 μL of cell samples. We further assessed the spheroids formed by the portable device and the impact of the acoustic field on spheroids and demonstrated the drug screening with assembled spheroids. More importantly, the integrated acoustic device can be further integrated with other components for more complex cell culture and all-round analysis. This portable and effective integrated device provides a new avenue for clinical biomedicine.

中文翻译：

用于培养 3D 细胞阵列的集成声学芯片

三维 (3D) 细胞阵列提供了一个体外临床药物筛选平台，但笨重的培养设备限制了其应用场景。在这里，我们展示了一种集成的便携式设备，可以实现 3D 细胞球体的无接触构建。便携式设备产生的超声波与毛细管之间的相互作用导致周期性压力节点或波腹，从而形成用于细胞培养的 3D 细胞阵列。这样的 3D 细胞阵列模式可以在几秒钟内构建，并且只需要 1 μL 的细胞样本。我们进一步评估了便携式设备形成的球体以及声场对球体的影响，并展示了组装球体的药物筛选。更重要的是，集成声学装置可以与其他组件进一步集成，以进行更复杂的细胞培养和全方位分析。

更新日期：2022-09-01

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