Gold (Au) and palladium (Pd) species have been widely used in synthetic chemistry, biology, and environmental sciences, raising hard-to-ignore health and environment risks due to their potential toxicity. Herein, a novel dual-functional fluorescent probe was rationally designed and developed for simultaneous quantification and visualization of Au³⁺ and Pd species in the environmental and biological systems. Significant fluorescence enhancement at 484 and 606 nm and color changes were observed upon addition of Au³⁺ and Pd⁰ with high sensitivity. The detection limit for Au³⁺ and Pd⁰ was as low as 68 nM and 7 nM, respectively. Combined with smartphone chromaticity and dual-channel fluorescence imaging analysis, this probe was successfully applied to the quantification and visualization of Au³⁺ and Pd species in real water samples. Thus, simultaneous naked-eye visualization and quantification of Au³⁺ and Pd⁰ in real water samples was achieved using this probe. The probe was also successfully applied to fluorescence imaging of Au³⁺ and Pd⁰ in HeLa cells and zebrafish, promoting the monitor and understanding about gold and palladium species in environmental and biological systems.

金 (Au) 和钯 (Pd) 物种已广泛用于合成化学、生物学和环境科学，由于其潜在的毒性而引发了难以忽视的健康和环境风险。在此，合理设计和开发了一种新型双功能荧光探针，用于同时定量和可视化环境和生物系统中的 Au ³⁺和 Pd 物种。添加高灵敏度的Au ³⁺和Pd ⁰后，在484和606 nm处观察到显着的荧光增强和颜色变化。Au ³⁺和 Pd ^{0的检测限}分别低至 68 nM 和 7 nM。结合智能手机色度和双通道荧光成像分析，该探针成功应用于真实水样中Au ³⁺和Pd 的定量和可视化。因此，使用该探针实现了真实水样中Au ³⁺和Pd ⁰的同时肉眼可视化和量化。该探针还成功应用于HeLa细胞和斑马鱼中Au ³⁺和Pd ⁰的荧光成像，促进了对环境和生物系统中金和钯物种的监测和认识。