基于细胞膜和 V2C MXene 的电化学免疫传感器，具有增强的抗污染能力，用于检测 CD44,ACS Sensors

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基于细胞膜和 V2C MXene 的电化学免疫传感器，具有增强的抗污染能力，用于检测 CD44
ACS Sensors ( IF 8.2 ) Pub Date : 2022-08-30 , DOI: 10.1021/acssensors.2c01215
Meiling Lian ₁ , Yuqing Shi ₁ , Liuxing Chen ₁ , Yongji Qin ₂ , Wei Zhang ₁ , Jingbo Zhao ₁ , Da Chen ₁

Affiliation

生物分子在电化学生物传感器中的非活性吸附和干扰是一个备受关注的话题。直接利用天然细胞膜赋予电化学表面防污和生物相容性特征是一种很有前途的策略，而不是试图合成复制复杂的生物界面特性。_{在这项研究中，我们通过血小板膜/Au 纳米颗粒/分层 V 2} C 纳米片 (PM/AuNPs/dV ₂ C)-修饰电极作为传感界面和亚甲基蓝/胺化的底物，提出了一种面部和敏感的三明治型防污免疫分析。金属有机骨架 (MB@NH ₂ -Fe-MOF-Zn) 作为电化学信号探针。该生物传感器完美结合了载有 AuNPs 的 V 的高电导率₂ C MXene 具有优异的NH _{2负载性能}-Fe-MOF-Zn 提高电化学传感性能。此外，均质细胞膜优异的防污性能可有效防止模型蛋白的非特异性吸附。获得的防污生物传感器具有超灵敏检测复杂液体中CD44和CD44阳性癌细胞的能力，对CD44的分析具有良好的分析性能，线性范围为0.5 ng/mL至500 ng/mL。这种开发具有增强防污能力的基于细胞膜的生物传感系统的策略可以很容易地扩展到其他复杂生物传感器的构建，先进的生物探针和分析方法为准确量化与肿瘤进展相关的生物标志物提供了有利的手段。

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Cell Membrane and V2C MXene-Based Electrochemical Immunosensor with Enhanced Antifouling Capability for Detection of CD44

The inactive adsorption and interference of biomolecules in electrochemical biosensors is a topic of intense interest. Directly utilizing native cell membranes to endow electrochemical surfaces with antifouling and biocompatible features is a promising strategy, rather than attempting to synthetically replicate complex biological interface properties. In this study, we present a facial and sensitive sandwich-type antifouling immunoassay through platelet membrane/Au nanoparticle/delaminated V₂C nanosheet (PM/AuNPs/d-V₂C)-modified electrode as the substrate of sensing interface and methylene blue/aminated metal organic framework (MB@NH₂-Fe-MOF-Zn) as an electrochemical signal probe. The biosensor perfectly integrates the high conductivity of AuNPs-loaded V₂C MXene with the excellent loading property of NH₂-Fe-MOF-Zn to improve the electrochemical sensing performance. In addition, the excellent antifouling properties of the homogeneous cell membrane can effectively prevent the non-specific adsorption of model proteins. The obtained antifouling biosensor possesses the capability of ultrasensitive detection of CD44 and CD44-positive cancer cell in complex liquids and exhibits good analytical performance for the analysis of CD44 with a linear range from 0.5 ng/mL to 500 ng/mL. This strategy of developing cell membrane-based biosensing systems with enhanced antifouling capability can be easily expanded to the construction of other complex biosensors, and the advanced biological probes and analytical methods provide a favorable means to accurately quantify biomarkers associated with tumor progression.

更新日期：2022-08-30

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