The upscaling of pea protein extraction from laboratory scale with a centrifuge to pilot scale with a decanter centrifuge was investigated, and the pea protein extraction efficiency from dry milled and pre-treated peas was compared. Upscaling from laboratory to pilot scale is possible since starch was under the limit of detection (< 0.5%). The protein banding pattern of a sodium-dodecyl-sulfate polyacrylamide gel electrophoresis confirmed that albumins and globulins were extracted by alkali extraction. Protein yield increased from 59.5% to 67.1% for dry milled peas due to constant and quick discharge of dry matter in the decanter centrifuge. For pre-treated peas, the protein yield increased from 60.3% to 94.3%, which is explained by an improved cutting and improved separation in pilot scale compared to laboratory scale. The impact of acceleration, mass flow, differential speed and their respective interactions in the decanting process was determined with a design of experiments. For dry milled peas, only the mass flow exceeded the significance level. However, a mass flow of 5 kg h⁻¹, an acceleration of 1000 g\(\times\) and a differential speed of 50 min⁻¹ led to the highest protein yield of 75.6%. The obtained protein yields for the pre-treated peas were in the range of 83 to 96% and therefore did not show significant differences in protein yield.

研究了使用离心机从实验室规模到使用卧螺离心机中试规模的豌豆蛋白提取升级，并比较了从干磨和预处理豌豆中提取豌豆蛋白的效率。由于淀粉低于检测限（< 0.5%），因此可以从实验室扩大到中试规模。十二烷基硫酸钠聚丙烯酰胺凝胶电泳的蛋白质条带图证实白蛋白和球蛋白是通过碱提取法提取的。由于卧螺离心机中干物质的持续快速排放，干磨豌豆的蛋白质产量从 59.5% 增加到 67.1%。对于预处理的豌豆，蛋白质产量从 60.3% 提高到 94.3%，这可以通过与实验室规模相比，中试规模改进的切割和改进的分离来解释。通过实验设计确定了倾析过程中加速度、质量流量、差速及其各自相互作用的影响。对于干磨豌豆，只有质量流量超过显着性水平。然而，质量流量为 5 kg h^-1，1000 g \(\times\)的加速度和 50 分钟的差速^-1导致最高的蛋白质产量为 75.6%。预处理豌豆获得的蛋白质产量在 83% 至 96% 的范围内，因此蛋白质产量没有显着差异。