The aminoshikimate pathway of formation of 3-amino-5-hydroxybenzoic acid (AHBA), the precursor of ansamycin and other antibiotics is reviewed. In this biosynthesis, genes for kanosamine formation have been recruited from other genomes, to provide a nitrogenous precursor. Kanosamine is then phosphorylated and converted by common cellular enzymes into 1-deoxy-1-imino-erythrose 4-phosphate, the substrate for the formation of aminoDAHP. This is converted via 5-deoxy-5-aminodehydroquinic acid and 5-deoxy-5-aminodehydroshikimic acid into AHBA. Remarkably, the pyridoxal phosphate enzyme AHBA synthase seems to have two catalytic functions: As a homodimer, it catalyzes the last reaction in the pathway, the aromatization of 5-deoxy-5-aminodehydroshikimic acid, and at the beginning of the pathway in a complex with the oxidoreductase RifL it catalyzes the transamination of UDP-3-keto-D-glucose. The AHBA synthase gene also serves as a useful tool in the genetic screening for new ansamycins and other AHBA-derived natural products.

中文翻译：

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3-氨基-5-羟基苯甲酸（AHBA）的生物合成，这是安沙霉素和丝裂霉素抗生素中mC7N单元的前体。

综述了3-氨基-5-羟基苯甲酸（AHBA），安沙霉素和其他抗生素的前体形成的氨基shi酸酯途径。在这种生物合成中，已从其他基因组中招募了形成甘露胺的基因，以提供含氮前体。然后将Kanosamine磷酸化，并通过常见的细胞酶将其转化为1-deoxy-1-imino-erythrose 4-phosphate（形成氨基DAHP的基质）。经由5-脱氧-5-氨基脱氢奎宁酸和5-脱氧-5-氨基脱氢hydro草酸将其转化为AHBA。值得注意的是，吡ido醛磷酸酶AHBA合酶似乎具有两个催化功能：作为同型二聚体，它催化该途径中的最后一个反应，即5-脱氧-5-氨基脱氢shi草酸的芳构化，在与氧化还原酶RifL形成复合物的途径开始时，它催化UDP-3-酮-D-葡萄糖的转氨作用。AHBA合酶基因在新安沙霉素和其他AHBA衍生的天然产物的遗传筛选中也可作为有用的工具。