A Dual-Mode NADH Biosensor Based on Gold Nanostars Decorated CoFe2 Metal–Organic Frameworks to Reveal Dynamics of Cell Metabolism,ACS Sensors

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A Dual-Mode NADH Biosensor Based on Gold Nanostars Decorated CoFe2 Metal–Organic Frameworks to Reveal Dynamics of Cell Metabolism
ACS Sensors ( IF 8.2 ) Pub Date : 2022-08-24 , DOI: 10.1021/acssensors.2c01175
Xiaoping Zhao ₁ , Ruoxin Niu ₁ , Shu Fan ₁ , Xunan Jing ₂ , Rui Gao ₁ , Hongbo Yang ₁ , Heng Wang ₁ , Daquan Wang ₁ , Zhiwei Yang ₃ , Yunchuan Xie ₁ , Junjun She ₂ , Peng Chen ₄ , Lingjie Meng _{1,

2,

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Affiliation

Nicotinamide adenine dinucleotide (NADH) is central to metabolism and implicated in various diseases. Herein, nanohybrids of gold nanostars and metal–organic frameworks are devised and demonstrated as a dual-mode NADH sensor, for which colorimetric detection is enabled by its peroxidase-like nanozyme property and Raman detection is realized by its surface-enhanced Raman scattering property with the detection limit as low as 28 pM. More importantly, this probe enables real-time SERS monitoring in living cells, providing a unique tool to investigate dynamic cellular processes involving NADH. Our experiments reveal that metabolism dynamics is accelerated by glucose and is much higher in cancerous cells. The SERS results can also be verified by the colorimetric detection. This sensor provides a new potential to detect biomarkers and their dynamics in situ.

中文翻译：

基于金纳米星装饰 CoFe2 金属有机框架的双模式 NADH 生物传感器揭示细胞代谢动力学

烟酰胺腺嘌呤二核苷酸 (NADH) 是新陈代谢的核心，与多种疾病有关。在此，金纳米星和金属有机框架的纳米杂化物被设计并展示为双模式 NADH 传感器，其比色检测通过其类似过氧化物酶的纳米酶特性实现，拉曼检测通过其表面增强拉曼散射特性实现检测限低至 28 pM。更重要的是，该探针能够在活细胞中进行实时 SERS 监测，为研究涉及 NADH 的动态细胞过程提供了独特的工具。我们的实验表明，代谢动力学被葡萄糖加速，并且在癌细胞中要高得多。SERS 结果也可以通过比色检测来验证。该传感器提供了检测生物标志物及其动态的新潜力原地。

更新日期：2022-08-24

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