自发膨胀、电成形、凝胶辅助和相转移方法制备的巨型囊泡膜的弯曲刚度、电容和剪切粘度：比较研究,Langmuir

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自发膨胀、电成形、凝胶辅助和相转移方法制备的巨型囊泡膜的弯曲刚度、电容和剪切粘度：比较研究
Langmuir ( IF 3.7 ) Pub Date : 2022-08-22 , DOI: 10.1021/acs.langmuir.2c01402
Hammad A Faizi ₁ , Annie Tsui ₂ , Rumiana Dimova ₃ , Petia M Vlahovska ₄

Affiliation

巨型单层囊泡（GUV）形式的封闭脂质双层是常用的膜模型。人们已经开发了多种方法来制备 GUV，但尚不清楚是否所有方法都能产生具有相同弹性、电学和流变特性的膜。在这里，我们结合 GUV 的闪烁光谱和电成型，在相同条件下测量棕榈酰油酰磷脂酰胆碱 (POPC) 膜的膜电容、弯曲刚度和剪切表面粘度，POPC 膜由几种常用的制备方法形成：薄膜水化（自发膨胀）、电成型、使用聚（乙烯醇）（PVA）或琼脂糖进行凝胶辅助溶胀，以及相转移。我们发现除琼脂糖水合方法外，所有方法的弯曲刚度值都相对相似。此外，除了通过 PVA 凝胶水合制备的囊泡外，电容值相似。有趣的是，与电成型和自发膨胀相比，通过凝胶辅助和相转移方法制备的膜表现出更高的剪切粘度，这可能是由于脂质双层结构中残留有聚合物（PVA 和琼脂糖）和油（十六烷和矿物）。

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Bending Rigidity, Capacitance, and Shear Viscosity of Giant Vesicle Membranes Prepared by Spontaneous Swelling, Electroformation, Gel-Assisted, and Phase Transfer Methods: A Comparative Study

Closed lipid bilayers in the form of giant unilamellar vesicles (GUVs) are commonly used membrane models. Various methods have been developed to prepare GUVs, however it is unknown if all approaches yield membranes with the same elastic, electric, and rheological properties. Here, we combine flickering spectroscopy and electrodefomation of GUVs to measure, at identical conditions, membrane capacitance, bending rigidity and shear surface viscosity of palmitoyloleoylphosphatidylcholine (POPC) membranes formed by several commonly used preparation methods: thin film hydration (spontaneous swelling), electroformation, gel-assisted swelling using poly(vinyl alcohol) (PVA) or agarose, and phase-transfer. We find relatively similar bending rigidity value across all the methods except for the agarose hydration method. In addition, the capacitance values are similar except for vesicles prepared via PVA gel hydration. Intriguingly, membranes prepared by the gel-assisted and phase-transfer methods exhibit much higher shear viscosity compared to electroformation and spontaneous swelling, likely due to remnants of polymers (PVA and agarose) and oils (hexadecane and mineral) in the lipid bilayer structure.

更新日期：2022-08-22

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