通过定期光热治疗调节巨噬细胞极化以促进成骨,Small

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通过定期光热治疗调节巨噬细胞极化以促进成骨
Small ( IF 13.0 ) Pub Date : 2022-08-19 , DOI: 10.1002/smll.202202691
Bo Li ₁ , Fuli Liu ₁ , Jing Ye ₁ , Xinmei Cai ₁ , Runliu Qian ₁ , Kaiwang Zhang ₂ , Yufeng Zheng ₃ , Shuilin Wu ₃ , Yong Han _{1,

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Affiliation

丰富的活性氧（ROS）及其在骨损伤部位产生的过度炎症阻碍了骨内钛基植入物的成骨。在此，抗氧化聚多巴胺（PDA）沉积在钛上水热生长形成的羟基磷灰石（HA）纳米棒上，形成以HA为核、PDA为无定形壳的核壳结构纳米棒状阵列（PDA@HA），不仅显示了 ROS 清除能力，而且显示了近红外 (NIR) 光衍生的光热效应。PDA@HA 基于其 ROS 清除能力在一定程度上抑制炎症，而温和温度（41 ± 1 °C）下的周期性光热处理（PTT）进一步加速粘附在 PDA@ 上的巨噬细胞（MΦs）的转变HA 在体外和体内从促炎 (M1) 表型到抗炎 (M2) 表型。转录组学分析表明，PI3K-Akt1 信号通路的激活是周期性 PTT 诱导的 MΦ 的 M1 到 M2 转变加速的原因。具有 M2 巨噬细胞旁分泌细胞因子的间充质干细胞 (MSCs)，具有温和 PTT 的 PDA@HA 极大地促进了 MSCs 的成骨功能，从而促进成骨。这项工作为采用温和周期性 PTT 诱导有利的成骨免疫调节微环境铺平了道路，并提供了对其潜在免疫调节机制的见解。具有轻度 PTT 的 PDA@HA 极大地促进了 MSCs 的成骨功能，从而促进成骨。这项工作为采用温和周期性 PTT 诱导有利的成骨免疫调节微环境铺平了道路，并提供了对其潜在免疫调节机制的见解。具有轻度 PTT 的 PDA@HA 极大地促进了 MSCs 的成骨功能，从而促进成骨。这项工作为采用温和周期性 PTT 诱导有利的成骨免疫调节微环境铺平了道路，并提供了对其潜在免疫调节机制的见解。

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Regulation of Macrophage Polarization Through Periodic Photo-Thermal Treatment to Facilitate Osteogenesis

The richened reactive oxygen species (ROS) and their derived excessive inflammation at bone injured sites hinder osteogenesis of endosseous Ti-based implants. Herein, anti-oxidized polydopamine (PDA) is deposited on hydrothermal growth formed hydroxyapatite (HA) nanorods on Ti to form a core-shell structural nanorod-like array with HA as a core and PDA as an amorphous shell (PDA@HA), showing not only ROS scavenging ability but also near-infrared (NIR) light derived photo-thermal effects. PDA@HA suppresses inflammation based on its ROS scavenging ability to a certain extent, while periodic photo-thermal treatment (PTT) at a mild temperature (41 ± 1 °C) further accelerates the transition of the macrophages (MΦs) adhered to PDA@HA from the pro-inflammatory (M1) phenotype to the anti-inflammatory (M2) phenotype in vitro and in vivo. Transcriptomic analysis reveals that the activation of the PI3K-Akt1 signaling pathway is responsible for the periodic PTT induced acceleration of the M1-to-M2 transition of MΦs. Acting on mesenchymal stem cells (MSCs) with paracrine cytokines of M2 macrophages, PDA@HA with mild PTT greatly promote the osteogenetic functions of MSCs and thus osteogenesis. This work paves a way of employing mildly periodic PTT to induce a favorable immunomodulatory microenvironment for osteogenesis and provides insights into its underlying immunomodulation mechanism.

更新日期：2022-08-19

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