Lysophosphatidylcholine (LPC), a major phospholipid component of atherogenic lipoproteins, induces apoptosis of vascular smooth muscle cells and promotes arteriosclerosis. We developed both a solution-type colorimetric sensor and a fluorescence-based microfluidic gradient sensor that rapidly analyzes LPC in solution at the level of a few tens of micromolar. Zwitterionic polydiacetylene (PDZ) vesicles displayed a blue to purple-red color change and a fluorescence turn-on feature upon interaction with LPC as a result of the electrostatic and hydrophobic interaction between LPC and PDZ vesicles. The zwitterionic PDZ sensor system also displayed a remarkable substrate selectivity for LPC among various structural analogues. Significantly, we developed a multichannel microfluidic gradient sensor and on-chip fluorescence detection technique that can be utilized to simultaneously measure the LPC concentrations of seven solution gradients under flowing conditions within 10 min. The analytical system developed in this study is expected to be useful as a rapid in vitro diagnostic sensor for LPC, a biomarker for assessing the risk of developing arteriosclerosis.

溶血磷脂酰胆碱 (LPC) 是致动脉粥样硬化脂蛋白的主要磷脂成分，可诱导血管平滑肌细胞凋亡并促进动脉硬化。我们开发了溶液型比色传感器和基于荧光的微流体梯度传感器，可在几十微摩尔水平上快速分析溶液中的 LPC。由于 LPC 和 PDZ 囊泡之间的静电和疏水相互作用，两性离子聚二乙炔 (PDZ) 囊泡在与 LPC 相互作用时显示出蓝色至紫红色的颜色变化和荧光开启特征。两性离子 PDZ 传感器系统在各种结构类似物中也显示出对 LPC 的显着底物选择性。显著地，我们开发了一种多通道微流控梯度传感器和片上荧光检测技术，可用于在 10 分钟内同时测量流动条件下七种溶液梯度的 LPC 浓度。本研究中开发的分析系统有望用作快速LPC的体外诊断传感器，一种用于评估动脉硬化风险的生物标志物。