MRS Advances Pub Date : 2022-08-18 , DOI: 10.1557/s43580-022-00322-2 Mai S Rashwan 1, 2 , Zeinab M Anwar 2 , Harihara Baskaran 1 , Heidi B Martin 1
|
Nanodiamond (ND) with nitrogen vacancy (NV−) color centers has emerged as an important material for quantum sensing and imaging. Fluorescent, carboxylated ND (140 nm) is investigated for the detection of dopamine (DA), caffeine (CA), and ascorbic acid (AA). Over a 200 nM range, DA and CA quenched the ND fluorescence by 7.1 and 9.8%, respectively. For AA, fluorescence was quenched (2.9%) at nM concentrations and enhanced at μM concentrations. The quenching fit well to Langmuir adsorption isotherms. For DA-CA mixtures, the CA at nM concentrations did not affect DA quenching but interfered when at μM concentrations. The DA at nM or μM concentrations lessened CA quenching. For DA-AA mixtures with DA at mM concentrations, AA quenched fluorescence throughout the nM and μM range, with increased quenching in the nM range. These studies support ND fluorescence modulation as a possible sensor modality for bioanalyte detection.
Graphical Abstract
中文翻译：
用于神经化学检测的纳米金刚石 NV− 中心的荧光调制
具有氮空位（ NV− ）色心的纳米金刚石（ND）已成为量子传感和成像的重要材料。研究了荧光羧化 ND (140 nm) 来检测多巴胺 (DA)、咖啡因 (CA) 和抗坏血酸 (AA)。在 200 nM 范围内，DA 和 CA 分别将 ND 荧光淬灭 7.1% 和 9.8%。对于 AA，荧光在 nM 浓度下猝灭 (2.9%)，在 μM 浓度下增强。猝灭非常符合朗缪尔吸附等温线。对于 DA-CA 混合物，nM 浓度的 CA 不会影响 DA 猝灭，但在 μM 浓度时会产生干扰。 nM 或 μM 浓度的 DA 减少了 CA 猝灭。对于 mM 浓度的 DA-AA 混合物，AA 在整个 nM 和 μM 范围内猝灭荧光，并在 nM 范围内增强猝灭。这些研究支持 ND 荧光调制作为生物分析物检测的可能传感器方式。
京公网安备 11010802027423号