Mepanipyrim is a fungicide against several plant pathogens. However, no metabolic details have been established in fungi, which is the most important biomass in the natural environment. Cunninghamella elegans is a well-known fungal species with its strong resemblance to the mammalian xenobiotic metabolism. In this study, the detailed metabolic pathways of mepanipyrim were investigated with C. elegans. Approximately 87% of mepanipyrim was removed within 12 h with concomitant accumulation of nine metabolites. Structures of the metabolites were fully or tentatively identified with GC-MS and ¹H NMR. To determine the possible role of representative oxidative enzymes, piperonyl butoxide and methimazole were treated, and the kinetic responses of mepanipyrim and its metabolites were measured. Dose-dependent inhibition of metabolism was observed with piperonyl butoxide, while methimazole also inhibited the metabolism less effectively. The results indicate the possible involvement of cytochrome P450 and flavin-dependent monooxygenase in mepanipyrim metabolism. Comprehensive metabolic pathways can be deduced from the detailed analysis of metabolite profiles in control and inhibitor assays.

中文翻译：

---

土壤真菌Cunninghamella elegans ATCC36112对一种杀菌剂Mepanipyrim的代谢。

Mepanipyrim是对几种植物病原体的杀真菌剂。但是，尚未确定真菌的代谢细节，真菌是自然环境中最重要的生物质。秀丽线虫是一种众所周知的真菌，与哺乳动物的异源生物代谢非常相似。在这项研究中，秀丽隐杆线虫研究了详细的代谢途径。在12小时内约有87％的甲哌啶y被去除，同时伴有9种代谢物的积累。代谢物的结构已通过GC-MS和¹进行了完全或初步的鉴定1 H NMR。为了确定代表性氧化酶的可能作用，对胡椒基丁醚和甲巯咪唑进行了处理，并测定了甲吡嘧啶及其代谢产物的动力学响应。胡椒基丁醇可观察到剂量依赖性的新陈代谢抑制作用，而甲巯咪唑也不能有效抑制新陈代谢。结果表明，细胞色素P450和黄素依赖性单加氧酶可能参与了甲基吡y胺的代谢。全面的代谢途径可以从对照和抑制剂试验中代谢物谱的详细分析中得出。