Euphorbia resinifera latex has been extensively utilized in traditional medicine due to its range of bioactivities. Chromatographic separations on silica gel of ethanol extract of E. resinifera latex led to the development of a new procedure for isolating resiniferatoxin (4) via dried E. resinifera latex and the identification of nine compounds. Among these, catechol (7), protocatechuic acid (8) and 3,4-dihydroxyphenylacetic acid (9), known phenolic compounds, were identified for the first time in E. resinifera latex. Herein we investigated the effects of major compounds of the latex of E. resinifera on the yeast Saccharomyces cerevisiae, on the growth of Aspergillus carbonarius, a widespread fungal contaminant, and on the breast cancer cell line MCF7 as well as on MCF10A normal breast cells. 12-deoxyphorbol-13-isobutyrate-20-acetate (2) had an inhibiting effect on the growth of A. carbonarius, and 7-p-metoxyphenylacetate-3,8,12-triacetate ingol (3) showed a negative effect on yeast cell growth and also a cytotoxic effect on breast cancer cell line MCF7, but not on MCF10A cells. Deglucosyl euphorbioside A (5) and euphorbioside A (6) showed a discoloration effect that was possibly related to mitochondrial functionality in yeast, and also cytotoxicity only on the cancer cell line that was tested. Interestingly, treatment of MCF7 cells with 7-p-metoxyphenylacetate-3,8,12-triacetate ingol (3) and deglucosyl euphorbioside A (5) not only led to a specific cytotoxic effect but also to the increase in the level of intracellular ROS.

中文翻译：

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大戟胶乳中二萜和双倍半萜的抗真菌和细胞毒活性

由于其广泛的生物活性，大戟树脂胶乳已被广泛用于传统医学中。E.resinifera胶乳乙醇提取物硅胶色谱分离导致开发了一种通过干燥E.resinifera胶乳分离树脂毒素 ( 4 )并鉴定九种化合物的新方法。其中，已知酚类化合物儿茶酚( 7 )、原儿茶酸( 8 )和3,4-二羟基苯乙酸( 9 )首次在树脂胶乳中鉴定。在这里，我们研究了E.resinifera胶乳的主要化合物对酵母的影响Saccharomyces cerevisiae，对炭黑曲霉（一种广泛存在的真菌污染物）的生长，对乳腺癌细胞系 MCF7 以及对 MCF10A 正常乳腺细胞的研究。12-deoxyphorbol-13-isobutyrate-20-acetate ( 2 ) 对A. carbonarius的生长有抑制作用，7 - p -metoxyphenylacetate-3,8,12-triacetate ingol ( 3 ) 对酵母有负面作用细胞生长和对乳腺癌细胞系 MCF7 的细胞毒性作用，但对 MCF10A 细胞没有。脱葡糖基大戟二糖苷 A ( 5 ) 和大戟二糖苷 A ( 6) 显示出可能与酵母中的线粒体功能有关的变色效应，以及仅对所测试的癌细胞系的细胞毒性。有趣的是，用 7- p -metoxyphenylacetate-3,8,12-triacetate ingol ( 3 ) 和去葡糖基大戟二糖苷 A ( 5 )处理 MCF7 细胞不仅会导致特定的细胞毒性作用，而且会导致细胞内 ROS 水平的增加.