Two intergenic spacers cpDNA barcoding regions were used to assess the genetic diversity and phylogenetic structure of a collection of 25 Prunus accessions. The trnH-psbA and trnL-trnF intergenic spacers were able to distinguish and identify only four Prunus species. The average aligned length was 316–352 bp and 701–756 bp for trnH-psbA and trnL-trnF, respectively. The overall evolutionary divergence was higher in trnH-psbA than trnL-trnF. The transition/transversion bias (R) recorded as 0.59 in trnL-trnF and 0.89 in trnH-psbA. The number of invariable sites, nucleotide diversity (Pi), and the average number of nucleotide differences (k) was higher in the trnH-psbA region. The trnL-trnF records was above the other region in the number of variable sites, number of singleton variable sites, and the parsimony informative sites. Phylogenetic relationships among the 25 accessions of Prunus species were investigated. Most of the different Prunus species clustered in a homogenized distribution in both regions, except for the plum (P. domestica) accession (African Rose) was assigned with the peach (P. persica) accessions. The two intergenic cpDNA trnH-psbA and trnL-trnF were able to distinguish and identify the four Prunus species accessions.

两个基因间间隔 cpDNA 条形码区域用于评估 25 个李属种质的遗传多样性和系统发育结构。trn H- psb A 和trn L- trn F 基因间间隔区只能区分和识别四种李属植物。trn H- psb A 和trn L- trn F的平均对齐长度分别为 316-352 bp 和 701-756 bp 。trn H -psb A的总体进化差异高于trn L -trn F。过渡/颠换偏差（R ) 在trn L -trn F中记录为 0.59 ，在trn H -psb A 中记录为 0.89。不变位点的数量、核苷酸多样性 (Pi) 和平均核苷酸差异数 (k) 在 trn H - psb中更高一个地区。trn L- trn F 记录在可变位点数、单变量位点数和简约信息位点方面均高于其他区域。研究了25 个李属种质之间的系统发育关系。除了李子（P. domestica ) 种质 (African Rose) 与桃(P. persica)种质一起分配。两个基因间 cpDNA trn H- psb A 和trn L- trn F 能够区分和鉴定四种李属种质。