积分球辅助共振同步光谱法用于量化材料双光束紫外-可见光吸收和散射消光,Analytical Chemistry

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积分球辅助共振同步光谱法用于量化材料双光束紫外-可见光吸收和散射消光
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-08-12 , DOI: 10.1021/acs.analchem.2c02037
Max Wamsley ₁ , Pathum Wathudura ₁ , Juan Hu ₂ , Dongmao Zhang ₁

Affiliation

积分球 (IS) 已广泛用于表征混浊样品中的光吸收。然而，将基于 IS 的样品吸收系数转换为用双光束紫外-可见分光光度计量化的紫外-可见吸光度具有挑战性。在这里，我们报告了一种积分球辅助共振同步 (ISARS) 光谱方法，该方法使用配备积分球附件的传统荧光分光光度计进行。提供了用于量化样品、溶剂和仪器基线 ISARS 强度谱的数学模型和实验程序。已经开发了一个三参数分析模型，用于关联基于 ISARS 的 UV-vis 吸光度和使用双光束仪器测量的吸光度。这种 ISARS 方法能够定量分离光吸收和散射对使用双光束紫外-可见分光光度计测量的样品紫外-可见消光光谱的贡献。该 ISARS 技术的示例应用通过一系列具有显着不同光学复杂性的代表性样品进行了演示，从近似纯吸收体、纯散射体到共振激发和检测条件下的同时光吸收体、散射体和发射体。

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Integrating-Sphere-Assisted Resonance Synchronous Spectroscopy for the Quantification of Material Double-Beam UV–Vis Absorption and Scattering Extinction

Integrating spheres (IS) have been used extensively for the characterization of light absorption in turbid samples. However, converting the IS-based sample absorption coefficient to the UV–vis absorbance quantified with a double-beam UV–vis spectrophotometer is challenging. Herein, we report an integrating-sphere-assisted resonance synchronous (ISARS) spectroscopy method performed with conventional spectrofluorometers equipped with an integrating-sphere accessory. Mathematical models and experimental procedures for quantifying the sample, solvent, and instrument-baseline ISARS intensity spectra were provided. A three-parameter analytical model has been developed for correlating the ISARS-based UV–vis absorbance and the absorbance measured with double-beam instruments. This ISARS method enables the quantitative separation of light absorption and scattering contribution to the sample UV–vis extinction spectrum measured with double-beam UV–vis spectrophotometers. Example applications of this ISARS technique are demonstrated with a series of representative samples differing significantly in their optical complexities, from approximately pure absorbers, pure scatterers, to simultaneous light absorbers, scatterers, and emitters under resonance excitation and detection conditions.

更新日期：2022-08-12

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