In this work, a UPLC-MS/MS method was developed for the determination of gypenoside A and gypenoside XLIX in rat plasma. For chromatographic separation, a UPLC BEH C18 column was employed, the mobile phase comprised acetonitrile: water (w/0.1% formic acid), and the elution time was 4 min. Detection of each compound was enabled by electrospray ionization in negative-ion mode, and quantitative analysis was enabled by operating in multiple reaction monitoring (MRM) mode by monitoring the transitions of m/z 897.5⟶403.3 for gypenoside A, m/z 1045.5⟶118.9 for gypenoside XLIX, and m/z 825.4⟶617.5 for the internal standard. The calibration curves for gypenoside A and gypenoside XLIX demonstrated excellent linearity (r > 0.995) over the range of 2–3000 ng/mL. The intraday and interday precisions of gypenoside A and gypenoside XLIX were within 14.9%, the intraday and interday accuracies ranged from 90.1% to 113.9%, the recoveries were all greater than 88.3%, and the matrix effect ranged from 87.1% to 94.1%. The developed method was successfully applied in the determination of the pharmacokinetics of gypenoside A and gypenoside XLIX. Gypenoside A and gypenoside XLIX had very short half-lives in rats, with oral t_1/2z of 1.4 ± 0.2 h and 1.8 ± 0.6 h, respectively, and low bioavailabilities (0.90% and 0.14%, respectively).

中文翻译：

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UPLC-MS/MS法测定大鼠血浆中绞股蓝皂苷A和绞股蓝皂苷XLIX及其药代动力学和生物利用度

在这项工作中，建立了一种 UPLC-MS/MS 方法，用于测定大鼠血浆中的绞股蓝皂苷 A 和绞股蓝皂苷 XLIX。色谱分离采用UPLC BEH C18色谱柱，流动相为乙腈：水（w/0.1%甲酸），洗脱时间为4 min。在负离子模式下通过电喷雾电离对每种化合物进行检测，并通过在多反应监测 (MRM) 模式下通过监测绞股蓝皂苷 A 的 m/z 897.5⟶403.3、m/z 1045.5⟶ 的离子对进行定量分析绞股蓝皂苷 XLIX 为 118.9，内标为 m/z 825.4⟶617.5。gypenoside A 和 gypenoside XLIX 的校准曲线表现出优异的线性 ( r > 0.995) 在 2–3000 ng/mL 范围内。gypenoside A和gypenoside XLIX的日间和日间精密度在14.9%以内，日间和日间精度在90.1%~113.9%之间，回收率均大于88.3%，基质效应在87.1%~94.1%之间。所建立的方法成功地应用于绞股蓝皂苷A和绞股蓝皂苷XLIX的药代动力学测定。Gypenoside A 和 gypenoside XLIX 在大鼠中的半衰期非常短，口服t _1/2z分别为 1.4 ± 0.2 h 和 1.8 ± 0.6 h，生物利用度低（分别为 0.90% 和 0.14%）。