Glioblastoma multiforme is the most aggressive astrocytes brain tumor. Glioblastoma cancer stem cells and hypoxia conditions are well-known major obstacles in treatment. Studies have revealed that non-coding RNAs serve a critical role in glioblastoma progression, invasion, and resistance to chemo-radiotherapy. The present study examined the expression levels of microRNAs (in normoxic condition) and long non-coding RNAs (in normoxic and hypoxic conditions) in glioblastoma stem cells treated with the HSV-G47∆. The expression levels of 43 miRNAs and 8 lncRNAs isolated from U251-GBM-CSCs were analyzed using a miRCURY LNA custom PCR array and a quantitative PCR assay, respectively. The data revealed that out of 43 miRNAs that only were checked in normoxic condition, the only 8 miRNAs, including miR-7–1, miR-let-7b, miR-130a, miR-137, miR-200b, miR-221, miR-222, and miR-874, were markedly upregulated. The expression levels of lncRNAs, including LEF1 antisense RNA 1 (LEF1-AS1), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), long intergenic non-protein coding RNA 470 (LINC00470), tumor suppressor candidate 7 (TUSC7), HOX transcript antisense RNA (HOTAIR), nuclear paraspeckle assembly transcript 1 (NEAT1), and X inactive specific transcript (XIST), were markedly downregulated in the hypoxic microenvironment, and H19-imprinted maternally expressed transcript (H19) was not observed to be dysregulated in this environment. Under normoxic conditions, LEF1-AS1, MALAT1, LINC00470, H19, HOTAIR, NEAT1, and XIST were downregulated and TUSC7 was not targeted by HSV-G47∆. Overall, the present data shows HSVG47Δ treatment deregulates non-coding RNA expression in GBM-CSC tumor microenvironments.

多形性胶质母细胞瘤是最具侵袭性的星形胶质细胞脑肿瘤。胶质母细胞瘤癌症干细胞和缺氧条件是治疗中众所周知的主要障碍。研究表明，非编码 RNA 在胶质母细胞瘤的进展、侵袭和对化学放射疗法的耐药性中起着关键作用。本研究检测了用 HSV-G47Δ 处理的胶质母细胞瘤干细胞中微小 RNA（在含氧量正常的条件下）和长链非编码 RNA（在含氧量正常和低氧条件下）的表达水平。使用 miRCURY LNA 定制 PCR 阵列和定量 PCR 分析分别分析了从 U251-GBM-CSC 分离的 43 种 miRNA 和 8 种 lncRNA 的表达水平。数据显示，在仅在常氧条件下检查的 43 种 miRNA 中，只有 8 种 miRNA，包括miR-7-1，miR-let-7b、miR-130a、miR-137、miR-200b、miR-221、miR-222和miR-874显着上调。lncRNA的表达水平，包括LEF1反义RNA 1（LEF1-AS1）、转移相关肺腺癌转录本1（MALAT1）、长基因间非蛋白编码RNA 470（LINC00470）、肿瘤抑制候选物7（TUSC7）、HOX转录本反义 RNA ( HOTAIR )、核副啄木鸟组装转录物 1 ( NEAT1 ) 和 X 失活特异性转录物 ( XIST), 在低氧微环境中显着下调，并且没有观察到 H19 印记的母系表达转录物 ( H19 ) 在这种环境中失调。在含氧量正常的条件下，LEF1-AS1、MALAT1、LINC00470、H19、HOTAIR、NEAT1和XIST被下调，而TUSC7不是 HSV-G47Δ 的目标。总体而言，目前的数据显示 HSVG47Δ 治疗解除了 GBM-CSC 肿瘤微环境中非编码 RNA 表达的调节。