Resveratrol is a dietary polyphenol that interacts with gut microbiota to possess various biological activities. To identify the microbial metabolites of resveratrol, fresh feces from 12 volunteers were cultured in vitro. Their urine samples were collected after taking a commercial capsule containing 600 mg of resveratrol. Metabolites were characterized and quantified by UPLC-Q-Exactive plus orbitrap MS/MS. The results showed that dihydroresveratrol, 3-(4-hydroxyphenyl)-propionic acid, and lunularin were the major microbial metabolites of RSV with interindividual differences. 3-(4-Hydroxyphenyl)-propionic acid significantly attenuated the inflammatory response of LPS-treated RAW264.7 cells and DSS-induced colitis in antibiotics-treated pseudo-germ-free mice by regulating MAPK and NF-κB pathways. In contrast, dihydroresveratrol did not exhibit significant anti-inflammatory effects, and lunularin exhibited pro-inflammatory effects in cells. This study may help to better understand the health effects of resveratrol and its microbial metabolites.

白藜芦醇是一种膳食多酚，可与肠道微生物群相互作用，具有多种生物活性。为了鉴定白藜芦醇的微生物代谢物，对 12 名志愿者的新鲜粪便进行了体外培养。他们的尿液样本是在服用含有 600 毫克白藜芦醇的商业胶囊后收集的。通过 UPLC-Q-Exactive plus orbitrap MS/MS 对代谢物进行表征和量化。结果表明，二氢白藜芦醇、3-(4-羟基苯基)-丙酸和月桂醛是RSV的主要微生物代谢产物，存在个体差异。3-(4-Hydroxyphenyl)-propionic acid 通过调节 MAPK 和 NF-κB 通路显着减弱 LPS 处理的 RAW264.7 细胞的炎症反应和抗生素处理的假无菌小鼠中 DSS 诱导的结肠炎。相比之下，二氢白藜芦醇没有表现出显着的抗炎作用，而lunularin在细胞中表现出促炎作用。这项研究可能有助于更好地了解白藜芦醇及其微生物代谢物对健康的影响。