<br>源自人脐静脉内皮细胞分泌组的外泌体 (Exo-HUVEC) 可改善皮肤成纤维细胞的光老化,Clinical, Cosmetic and Investigational Dermatology

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源自人脐静脉内皮细胞分泌组的外泌体 (Exo-HUVEC) 可改善皮肤成纤维细胞的光老化
Clinical, Cosmetic and Investigational Dermatology ( IF 1.9 ) Pub Date : 2022-08-08 , DOI: 10.2147/ccid.s371330
Endra Yustin Ellistasari ₁ , Harijono Kariosentono ₂ , Bambang Purwanto ₃ , Brian Wasita ₄ , Risya Cilmiaty Arief Riswiyant ₅ , Eti Poncorini Pamungkasari ₆ , Soetrisno Soetrisno ₇

Affiliation

目的：这是一项体外实验研究，以UVB作为光老化模型，分析Exo-HUVEC对照射成纤维细胞的内皮细胞（CD31）、细胞增殖、基质金属蛋白酶1（MMP-1）和1型胶原的影响。

患者和方法：成纤维细胞培养物分为5组，即无UVB照射、UVB照射600mJ/cm ² 80秒作为光老化模型、UVB照射+Exo-HUVEC照射0.1%、0.5%和1%。内皮细胞CD31标记染色，ELISA检测MMP-1，MTT法检测细胞增殖情况；同时，使用流式细胞术测量1型胶原沉积和内皮细胞。

结果：本研究发现内皮细胞标志物CD31呈阳性。 UVB 照射对照组与 Exo-HUVEC 治疗组之间的细胞增殖、MMP-1 和 1 型胶原水平存在显着差异 (p < 0.05)。

结论： Exo-HUVEC 显着增加细胞增殖和 1 型胶原水平，同时降低受辐射成纤维细胞的 MMP-1 水平；因此，Exo-HUVEC 可改善皮肤成纤维细胞的光老化。
图文摘要：

关键词:光老化, Exo-HUVEC, 内皮细胞, 细胞增殖, MMP-1, 1型胶原

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Exosomes Derived from Secretome Human Umbilical Vein Endothelial Cells (Exo-HUVEC) Ameliorate the Photo-Aging of Skin Fibroblast

Purpose: This is an in-vitro experimental study to analyze the effect of Exo-HUVEC on endothelial cell (CD31), cell proliferation, matrix metalloproteinase 1 (MMP-1) and collagen type 1 on irradiated fibroblast with UVB as photo-aging model.
Patients and Methods: Fibroblast cultures were divided into 5 groups, namely without UVB exposure, UVB exposure 600mJ/cm² for 80 seconds as photo-aging model, and UVB exposure +Exo-HUVEC exposure 0.1%, 0.5% and 1%. The endothelial cell was stained with a CD31 marker, MMP-1 were examined with ELISA, cell proliferation is detected using an MTT assay; meanwhile, collagen type 1 deposition and endothelial cell were measured using flowcytometry.
Results: This study found positive endothelial cell marker CD31. Significant difference was found in cell proliferation, MMP-1 and collagen type 1 level between the control group with UVB irradiation and the treatment group with Exo-HUVEC (p < 0.05).
Conclusion: Exo-HUVEC significantly increases cell proliferation and collagen type 1 level, while decrease MMP-1 levels on irradiated fibroblast; therefore, Exo-HUVEC ameliorate the photo-aging of skin fibroblast.
Graphical Abstract:

Keywords: photo-aging, Exo-HUVEC, endothelial cell, cell proliferation, MMP-1, collagen type 1

更新日期：2022-08-08

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