Six coumarin derivatives including two new, 2-acetyl-4-hydroxy-6H-furo[2,3-g]chromen-6-one (1) and 2-(1',2'-dihydroxypropan-2'-yl)-4-hydroxy-6H-furo[2,3-g]chromen-6-one (2), and four known ones, 7H-furo [3,2-g][1]benzopyran-7-one (3), esculetin (4), isoscopoletin (5) and 7,8-dihydroxy-6-methoxycoumarin (6), were isolated for the first time from the whole plant of Spermacoce latifolia. Their structures were determined by detailed spectroscopic analysis and comparison with literature reported data. In vitro antibacterial assay indicated that compounds 1, 2 and 4 were active toward bacteria Staphyloccocus aureus, Bacillus subtilis and Bacillus cereus with MIC values ranging from 7.81 to 62.5 ug/mL.

六种香豆素衍生物，包括两种新的 2-acetyl-4-hydroxy-6 H - furo[2,3 - g ]chromen-6-one ( 1 ) 和 2-(1',2'-dihydroxypropan-2'-yl )-4-hydroxy-6 H -furo[2,3- g ]chromen-6-one ( 2 )，以及四种已知的 7 H -furo [3,2- g ][1]benzopyran-7-one ( 3 )、七叶苷( 4 )、异东莨菪碱( 5 )和7,8-二羟基-6-甲氧基香豆素( 6 )为首次从阔叶草全株中分离得到。它们的结构是通过详细的光谱分析和与文献报道的数据进行比较来确定的。体外抗菌试验表明化合物1、2和4对细菌金黄色葡萄球菌、枯草芽孢杆菌和蜡状芽孢杆菌具有活性，MIC值范围为7.81至62.5 ug/mL。