The extensive use of perfluorooctanoic acid (PFOA), and its substitute hexafluoropropylene oxide trimer acid (HFPO-TA) has resulted in their frequent detection in environmental samples. However, little is known of their bioavailability via oral ingestion and the influence of food co-ingestion on absorption. Here, the relative bioavailability (RBA) of PFOA and HFPO-TA in soil was measured using an in vivo mouse model in the presence of food with different nutritional statuses (n = 11). PFOA and HFPO-TA RBA in soil was variable depending on nutrient co-administration, ranging from 29.8–95.5 % and 43.9–68.0 %, respectively. For both PFOA and HFPO-TA, a significantly negative correlation was observed between RBA and protein content in food (r = 0.57–0.72), while a positive correlation was observed with carbohydrate content (r = 0.51–0.57). Mechanistic studies showed that protein in food decreased PFOA and HFPO-TA RBA by down-regulating the expression of fatty acid binding protein 1 (FABP1) and up-regulating the expression of multidrug resistance associated protein 4 (Mrp4) in the liver, which are responsible for the absorption and efflux of PFOA and HFPO-TA. Dietary carbohydrates promoted albumin synthesis and up-regulated FABP1 expression thereby enhancing absorption and increasing PFOA and HFPO-TA RBA. This study provides an insight into potential dietary strategies for reducing exposure to per- and polyfluoroalkyl substances.

全氟辛酸 (PFOA) 及其替代品六氟环氧丙烷三聚酸 (HFPO-TA) 的广泛使用导致它们在环境样品中被频繁检测到。然而，人们对它们通过口服摄入的生物利用度以及食物共同摄入对吸收的影响知之甚少。在此，使用体内小鼠模型在不同营养状态的食物存在下测量土壤中 PFOA 和 HFPO-TA 的相对生物利用度 (RBA) (n = 11)。土壤中的 PFOA 和 HFPO-TA RBA 根据养分的共同施用而变化，范围分别为 29.8-95.5% 和 43.9-68.0%。对于 PFOA 和 HFPO-TA，RBA 与食品中蛋白质含量呈显着负相关（r = 0.57–0.72），而与碳水化合物含量呈正相关（r = 0.51–0.57）。机制研究表明，食物中的蛋白质通过下调肝脏中脂肪酸结合蛋白1（FABP1）的表达和上调多药耐药相关蛋白4（Mrp4）的表达来降低PFOA和HFPO-TA RBA，这些蛋白是负责 PFOA 和 HFPO-TA 的吸收和流出。膳食碳水化合物促进白蛋白合成并上调 FABP1 表达，从而增强吸收并增加 PFOA 和 HFPO-TA RBA。这项研究提供了对减少全氟烷基和多氟烷基物质暴露的潜在饮食策略的深入了解。