用单壁碳纳米管导电聚合物纳米复合材料修饰的一次性免疫传感器对钙网蛋白的阻抗检测,ACS Biomaterials Science & Engineering

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用单壁碳纳米管导电聚合物纳米复合材料修饰的一次性免疫传感器对钙网蛋白的阻抗检测
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2022-08-03 , DOI: 10.1021/acsbiomaterials.2c00499
Elif Burcu Aydın ₁ , Muhammet Aydın ₁ , Mustafa Kemal Sezgintürk ₂

Affiliation

首次利用电化学阻抗谱分析技术构建了无标记阻抗免疫传感系统，用于超灵敏测定人血清样品中钙网蛋白 (CALR) 生物标志物。新的生物传感器制造程序包括单壁碳纳米管 (SWCNT) 的电沉积，将 oxiran-2-yl 甲基 3-(1H-pyrrol-1-yl) 丙酸酯单体 ( Pepx ) 聚合到低成本和一次性的氧化铟锡 (ITO) 电极。单壁碳纳米管-PPepx在一步制造过程之后，在 ITO 上制备了纳米复合层。通过电化学分析和形态学监测技术对免疫传感器的制造过程和抗 CALR 与 CALR 之间的特征性生物分子相互作用进行了表征。在最佳条件下，所提出的生物传感器在 0.015–60 pg/mL 的检测范围内对 CALR 浓度呈线性响应，并且具有非常低的检测限 (4.6 fg/mL) 和良好的灵敏度 (0.43 kΩ pg ^–1 mL )厘米^–2). 通过成功量化人血清中的 CALR 水平，研究了生物传感器系统在临床分析中的可靠性。此外，通过使用 Dixon、Grubbs、 T检验和F检验评估生物传感器的重复性和再现性结果。因此，所提出的生物传感器是科学、快速和成功分析人血清样品中 CALR 的一种有前途的方法。

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Impedimetric Detection of Calreticulin by a Disposable Immunosensor Modified with a Single-Walled Carbon Nanotube-Conducting Polymer Nanocomposite

A label-free impedimetric immunosensing system was constructed for ultrasensitive determination of the calreticulin (CALR) biological marker in human serum samples utilizing an electrochemical impedance spectroscopy analysis technique for the first time. The new biosensor fabrication procedure consisted of electrodeposition of single-walled carbon nanotubes (SWCNTs) incorporating polymerization of an oxiran-2-yl methyl 3-(1H-pyrrol-1-yl) propanoate monomer (Pepx) onto a low-cost and disposable indium tin oxide (ITO) electrode. The SWCNTs-PPepx nanocomposite layer was prepared onto the ITO after the one-step fabrication procedure. The fabrication procedure of the immunosensor and the characteristic biomolecular interactions between the anti-CALR and CALR were characterized by electrochemical analysis and morphological monitoring techniques. Under optimum conditions, the proposed biosensor was responsive to CALR concentrations over the detection ranges of 0.015–60 pg/mL linearly, and it had a very low detection limit (4.6 fg/mL) and a favorable sensitivity (0.43 kΩ pg^–1 mL cm^–2). The reliability of the biosensor system in clinical analysis was investigated by successful quantification of CALR levels in human serum. Moreover, the repeatability and reproducibility results of the biosensor were evaluated by using Dixon, Grubbs, T-test, and F-tests. Consequently, the proposed biosensor was a promising method for scientific, rapid, and successful analysis of CALR in human serum samples.

更新日期：2022-08-03

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