Six members of the gasdermin family are involved in various biological functions in malignant tumors. The present study aimed to perform a comprehensive analysis of gasdermin family genes in pan-cancer. Raw data was acquired from the genotype-tissue expression (GTEx) and the Cancer Genome Atlas. High inter-tumor heterogeneity in the expression between paracancerous and tumor tissues was observed across cancers. Survival analysis confirmed that the risk or protective effects of gasdermin family members on prognosis depended on the cancer types. The mutation frequency appeared to be high, and the mutation group had a worse prognosis. Besides, gasdermin family genes were associated with immune infiltrate subtypes, stromal and immune cell infiltration levels, TMB, MSI, immune checkpoint gene expression, and tumor stemness scores. Moreover, gasdermin family gene expressions affected the expressions of MMR genes and methyltransferases and could predict cancer cells sensitivity to chemotherapeutic drugs. Subsequently, the findings were double-checked in LIHC and PAAD. GSEA results indicated the gasdermin family genes mainly involved in tumor metabolism and immune microenvironment remodeling related signaling pathways. In conclusion, our findings confirmed that gasdermin family genes were potential therapeutic cancer targets in pan-cancer.

gasdermin 家族的六个成员参与恶性肿瘤的各种生物学功能。本研究旨在对泛癌中的 gasdermin 家族基因进行全面分析。原始数据来自基因型组织表达 (GTEx) 和癌症基因组图谱。在癌症中观察到癌旁组织和肿瘤组织之间表达的高度肿瘤间异质性。生存分析证实，gasdermin 家族成员对预后的风险或保护作用取决于癌症类型。突变频率较高，突变组预后较差。此外，gasdermin 家族基因与免疫浸润亚型、基质和免疫细胞浸润水平、TMB、MSI、免疫检查点基因表达和肿瘤干性评分相关。而且，gasdermin家族基因表达影响MMR基因和甲基转移酶的表达，可以预测癌细胞对化疗药物的敏感性。随后，研究结果在 LIHC 和 PAAD 中进行了双重检查。GSEA结果表明gasdermin家族基因主要参与肿瘤代谢和免疫微环境重塑相关信号通路。总之，我们的研究结果证实，gasdermin 家族基因是泛癌中潜在的癌症治疗靶点。GSEA结果表明gasdermin家族基因主要参与肿瘤代谢和免疫微环境重塑相关信号通路。总之，我们的研究结果证实，gasdermin 家族基因是泛癌中潜在的癌症治疗靶点。GSEA结果表明gasdermin家族基因主要参与肿瘤代谢和免疫微环境重塑相关信号通路。总之，我们的研究结果证实，gasdermin 家族基因是泛癌中潜在的癌症治疗靶点。