Microchimica Acta ( IF 5.3 ) Pub Date : 2022-08-03 , DOI: 10.1007/s00604-022-05415-1 Jingnan Meng 1, 2 , Haoran Shen 2 , Jialin Chen 1 , Xing Shen 1 , Zeke Xu 1 , Juan Wang 1 , Yingju Liu 2 , Zhen-Lin Xu 1
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Due to the highly similar genetic background, it is difficult to distinguish Bacillus cereus (B. cereus) with other members of B. cereus group. Herein, an antibody-based colorimetric immunoassay using Cu-doped CeO2 nanospheres as peroxidase mimics was developed for the detection of B. cereus in food. First, monoclonal antibodies (mAbs) and polyclonal antibody (pAb) with good specificity to B. cereus were prepared and characterized. Second, the regular-shaped hollow Cu/CeO2 nanospheres with highly catalytic activity and biocompatibility were synthesized as mimic nanozymes to capture secondary antibody. Finally, a sandwich colorimetric immunoassay for the specific and sensitive detection of B. cereus was developed, showing linear detection range from 3.2 × 102 to 1 × 105 CFU/mL and a limit detection of 1.7 × 102 CFU/mL. The developed immunoassay holds great potential as an effective tool for detecting B. cereus in food poisoning.
Graphical abstract
中文翻译：
开发用于特异性筛选蜡状芽孢杆菌的 Cu 掺杂 CeO2 纳米球模拟基于纳米酶的免疫测定
由于遗传背景高度相似，很难将蜡状芽孢杆菌（B. cereus ）与蜡状芽孢杆菌组的其他成员区分开来。在此，开发了一种基于抗体的比色免疫测定法，使用 Cu 掺杂的 CeO 2纳米球作为过氧化物酶模拟物，用于检测食品中的蜡状芽孢杆菌。首先，制备并表征了对蜡状芽孢杆菌具有良好特异性的单克隆抗体（mAb）和多克隆抗体（pAb） 。二、规则形状的空心Cu/CeO 2合成具有高催化活性和生物相容性的纳米球作为模拟纳米酶来捕获二抗。最后，开发了一种用于特异性和灵敏检测蜡状芽孢杆菌的夹心比色免疫测定法，其线性检测范围为3.2×10 2至1×10 5 CFU/mL，限检测为1.7×10 2 CFU/mL。开发的免疫测定法作为检测食物中毒中蜡状芽孢杆菌的有效工具具有巨大潜力。
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