定量相位成像：生物医学的最新进展和扩展潜力,ACS Nano

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定量相位成像：生物医学的最新进展和扩展潜力
ACS Nano ( IF 15.8 ) Pub Date : 2022-08-02 , DOI: 10.1021/acsnano.1c11507
Thang L. Nguyen , Soorya Pradeep , Robert L. Judson-Torres , Jason Reed , Michael A. Teitell , Thomas A. Zangle

定量相位成像 (QPI) 是一种无标记的宽视场显微镜方法，在生物医学应用中具有重要的机会。QPI 利用光穿过哺乳动物细胞等透明物体时的自然相移来量化生物量分布以及生物量的时空变化。据 60 多年前的细胞研究报道，QPI 硬件和软件的不断进步正在导致生物学领域的众多应用，并且在过去二十年中其实用性急剧扩大。如今，对细胞大小、形态、行为、细胞粘弹性、药物功效、生物量积累和周转以及运输力学的研究正在支持对发育、生理学、神经活动、癌症以及其他生理过程和疾病的研究。在这里，我们从基本原理开始回顾生物学中的 QPI 领域，然后讨论当前可用或正在开发的技术方法，最后检查正在使用或正在开发的应用程序的广度。我们评论了在关键生物医学环境中部署 QPI 的优点和缺点，并总结了基于将 QPI 与其他方法相结合而出现的新挑战和机遇，进一步扩大了 QPI 的范围和实用性。

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Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine

Quantitative phase imaging (QPI) is a label-free, wide-field microscopy approach with significant opportunities for biomedical applications. QPI uses the natural phase shift of light as it passes through a transparent object, such as a mammalian cell, to quantify biomass distribution and spatial and temporal changes in biomass. Reported in cell studies more than 60 years ago, ongoing advances in QPI hardware and software are leading to numerous applications in biology, with a dramatic expansion in utility over the past two decades. Today, investigations of cell size, morphology, behavior, cellular viscoelasticity, drug efficacy, biomass accumulation and turnover, and transport mechanics are supporting studies of development, physiology, neural activity, cancer, and additional physiological processes and diseases. Here, we review the field of QPI in biology starting with underlying principles, followed by a discussion of technical approaches currently available or being developed, and end with an examination of the breadth of applications in use or under development. We comment on strengths and shortcomings for the deployment of QPI in key biomedical contexts and conclude with emerging challenges and opportunities based on combining QPI with other methodologies that expand the scope and utility of QPI even further.

更新日期：2022-08-02

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