含有蓝藻的光捕获人工细胞用于 CO2 固定和进一步代谢模拟,Small

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含有蓝藻的光捕获人工细胞用于 CO2 固定和进一步代谢模拟
Small ( IF 13.0 ) Pub Date : 2022-07-29 , DOI: 10.1002/smll.202201305
Boyu Yang ₁ , Shubin Li ₁ , Wei Mu ₁ , Zhao Wang ₁ , Xiaojun Han ₁

Affiliation

自下而上构建的人造细胞有助于理解细胞工作机制，为生物体的进化提供线索。能量供应和代谢模拟是人造细胞领域的关键问题。在此，证明了含有能够收集光和固定二氧化碳的蓝藻的人造细胞通过将光能转化为化学能来产生葡萄糖分子。研究了从葡萄糖分子开始的两条下游“代谢”途径。一种涉及酶级联反应以产生 H ₂ O ₂首先（由葡萄糖氧化酶辅助），然后将 Amplex 红转化为试卤灵（由辣根过氧化物酶辅助）。另一条途径更具生物学相关性。葡萄糖分子脱氢以将氢转移到烟酰胺腺嘌呤二核苷酸 (NAD ⁺ )，以在葡萄糖脱氢酶存在下产生烟酰胺腺嘌呤二核苷酸氢化物 (NADH) 分子。进一步地，NADH分子在乳酸脱氢酶的催化下被丙酮酸氧化成NAD ⁺，同时得到乳酸。因此，建立了NADH/NAD ⁺的级联循环。这里建造的人造细胞为研究人造细胞内更复杂的能量供应代谢铺平了道路。

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Light-Harvesting Artificial Cells Containing Cyanobacteria for CO2 Fixation and Further Metabolism Mimicking

The bottom-up constructed artificial cells help to understand the cell working mechanism and provide the evolution clues for organisms. The energy supply and metabolism mimicry are the key issues in the field of artificial cells. Herein, an artificial cell containing cyanobacteria capable of light harvesting and carbon dioxide fixation is demonstrated to produce glucose molecules by converting light energy into chemical energy. Two downstream “metabolic” pathways starting from glucose molecules are investigated. One involves enzyme cascade reaction to produce H₂O₂ (assisted by glucose oxidase) first, followed by converting Amplex red to resorufin (assisted by horseradish peroxidase). The other pathway is more biologically relevant. Glucose molecules are dehydrogenated to transfer hydrogens to nicotinamide adenine dinucleotide (NAD⁺) for the production of nicotinamide adenine dinucleotide hydride (NADH) molecules in the presence of glucose dehydrogenase. Further, NADH molecules are oxidized into NAD⁺ by pyruvate catalyzed by lactate dehydrogenase, meanwhile, lactate is obtained. Therefore, the cascade cycling of NADH/NAD⁺ is built. The artificial cells built here pave the way for investigating more complicated energy-supplied metabolism inside artificial cells.

更新日期：2022-07-29

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