环境毒理学中单细胞毒物基因组学的进展,Environmental Science & Technology

当前位置： X-MOL 学术 › Environ. Sci. Technol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

环境毒理学中单细胞毒物基因组学的进展
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2022-07-26 , DOI: 10.1021/acs.est.2c01098
Yuxuan Liu ₁ , Ling Chen ₁ , Jing Yu ₁ , Lin Ye ₁ , Haidong Hu ₁ , Jinfeng Wang ₁ , Bing Wu ₁

Affiliation

由于新技术的应用，环境污染物的毒性评价体系发生了许多变化。单细胞毒物基因组学通过将我们的分析分辨率提高到单细胞水平，正在迅速改变我们对环境毒理学的看法。该技术在环境毒理学中的应用已经开始出现，并且正在迅速扩大现有技术和应用的组合。在此，我们首先总结了单细胞测序过程中单细胞分离和扩增所涉及的不同方法，比较了不同方法的优缺点，并分析了它们的发展趋势。然后，我们回顾了单细胞毒理学在环境毒理学方面的主要进展，重点分析了该技术在早期胚胎污染物靶细胞识别、细胞亚型对污染物的异质反应、未知微生物中致病菌等方面的应用前景，并突出了该方法高分辨率、高分辨力的独特之处。吞吐量和高特异性的例子。我们还预测了这项技术的进一步应用以及它在环境毒理学方面带来的革命。总体而言，这些进展将为控制或减轻威胁人类和生态系统健康的外源性毒理学效应提供切实可行的解决方案，有助于提高我们对受污染物影响的生理过程的理解，并导致新的污染控制方法的出现。

"点击查看英文标题和摘要"

Advances in Single-Cell Toxicogenomics in Environmental Toxicology

The toxicity evaluation system of environmental pollutants has undergone numerous changes due to the application of new technologies. Single-cell toxicogenomics is rapidly changing our view on environmental toxicology by increasing the resolution of our analysis to the level of a single cell. Applications of this technology in environmental toxicology have begun to emerge and are rapidly expanding the portfolio of existing technologies and applications. Here, we first summarized different methods involved in single-cell isolation and amplification in single-cell sequencing process, compared the advantages and disadvantages of different methods, and analyzed their development trends. Then, we reviewed the main advances of single-cell toxicogenomics in environmental toxicology, emphatically analyzed the application prospects of this technology in identifying the target cells of pollutants in early embryos, clarifying the heterogeneous response of cell subtypes to pollutants, and finding pathogenic bacteria in unknown microbes, and highlighted the unique characteristics of this approach with high resolution, high throughput, and high specificity by examples. We also offered a prediction of the further application of this technology and the revolution it brings in environmental toxicology. Overall, these advances will provide practical solutions for controlling or mitigating exogenous toxicological effects that threaten human and ecosystem health, contribute to improving our understanding of the physiological processes affected by pollutants, and lead to the emergence of new methods of pollution control.

更新日期：2022-07-26

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南