Lignin of high purity and structural integrity was isolated from the enzymatic residue of corn stover. Degradation of the lignin by laccase, lignin peroxidase, and manganese peroxidase was investigated. Structural changes in the lignin after degradation were characterized by scanning electron microscopy, nitrogen adsorption and Fourier transform infrared spectroscopy, and the enzymatic products were systematically analyzed by gas chromatography mass spectrometry. The highest percentage of lignin degradation was obtained with a mixture of three enzymes (25.79%): laccase (Lac), the starting enzyme of the mixed enzyme reaction, worked with lignin peroxidase (LiP), and manganese peroxidase (MnP) to further degrade lignin. This degradation destroyed the macromolecular structure of lignin, broke its key chemical bonds, and opened benzene rings, thus producing more acidic compounds. This study elucidated the concept of degrading lignin from corn stover using the Lac, LiP and MnP enzymes synergistically, thus providing a theoretical basis for the biodegradation of lignin.

从玉米秸秆的酶残留物中分离出高纯度和结构完整性的木质素。研究了漆酶、木质素过氧化物酶和锰过氧化物酶对木质素的降解。采用扫描电镜、氮吸附和傅里叶变换红外光谱表征降解后木质素的结构变化，采用气相色谱质谱系统对酶产物进行系统分析。使用三种酶的混合物 (25.79%) 获得最高百分比的木质素降解：漆酶 (Lac)，混合酶反应的起始酶，与木质素过氧化物酶 (LiP) 和锰过氧化物酶 (MnP) 一起进一步降解木质素。这种降解破坏了木质素的大分子结构，破坏了它的关键化学键，打开了苯环，从而产生更多的酸性化合物。本研究阐明了Lac、LiP和MnP酶协同降解玉米秸秆中木质素的概念，为木质素的生物降解提供了理论依据。