Microchimica Acta ( IF 5.3 ) Pub Date : 2022-07-25 , DOI: 10.1007/s00604-022-05374-7 Bai Li 1 , Minghui Wang 1 , Xin Gu 1 , Jiajia Chen 1 , Xindi Yang 1 , Xuejin Liu 2 , Kuoxi Xu 1
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The selective detection of hydrogen sulfide in physiological and pathological processes has gained substantial attention in recent years. However, the real-time detection of hydrogen sulfide remains an elusive goal. In this work, a new type of hemicyanidin-based fluorescent “turn-on” probe NTR-HS (Ex = 680 nm, Em = 760 nm) was developed to detected H2S in a very short time (3 min). The fluorescence quantum yield is 0.15 and accompanied with a noticeable color change from violet to blue that can be used to detect H2S in the range 1.04 × 10−7–4 × 10−5 M with a limit of detection of 1.04 x 10-7 M. The NTR-HS probe was also used for imaging of endogenous hydrogen sulfide and mitochondrial localization in HCT116 and HeLa cells. The detection mechanism was studied through fluorescence, UV–Vis, NMR, and mass analysis. Notably, the probe was successfully used to imaging H2S in mice and locating hydrogen sulfide in the large intestine of mice.
Graphical abstract
中文翻译：
一种基于半花青素的 NIR 荧光探针，用于检测 H2S 并在细胞和小鼠中进行成像研究
近年来，选择性检测生理和病理过程中的硫化氢引起了广泛关注。然而，硫化氢的实时检测仍然是一个难以实现的目标。在这项工作中，开发了一种新型的基于半花青素的荧光“开启”探针NTR-HS（Ex = 680 nm，Em = 760 nm），可在极短的时间内（3分钟）检测H 2 S。荧光量子产率为 0.15，并伴有明显的从紫色到蓝色的颜色变化，可用于检测1.04 × 10 -7 –4 × 10 -5 M 范围内的 H 2 S， 检测限为 1.04 x 10 -7M. NTR-HS 探针还用于 HCT116 和 HeLa 细胞中内源性硫化氢和线粒体定位的成像。通过荧光、UV-Vis、NMR和质量分析研究了检测机制。值得注意的是，该探针成功地用于小鼠体内的H 2 S成像和小鼠大肠中硫化氢的定位。
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