A high-performance immobilized bacterial strain coated with magnetic iron oxide nanoparticles was used for Basic Blue 41 azo dye (BB 41 dye) decolorization. To create the coated bacterial strain, Raoultella Ornithinolytica sp. was isolated and identified under the accession number KT213695, then coated with manufactured magnetic iron oxide nanoparticles. SEM and SEM–EDX were used to characterize the coated bacteria and validate its morphological structure formation. The coated Raoultella Ornithinolytica sp. A1 (coated A1) generated a 95.20% decolorization for BB 41 dye at 1600 ppm starting concentration with an optimal dose of coated A1 5 mL/L, pH 8, under static conditions for 24 h at 37 °C. Continuous batch cycles were used, with BB 41 dye (1600 ppm) added every 24 h four times, to achieve a high decolorization efficiency of 80.14%. Furthermore, the metabolites of BB 41 dye biodegradation were investigated by gas chromatographic-mass spectrum analysis (GC–MS) and showed a less toxic effect on the bioindicator Artemia salina. Additionally, 5 mL/L of coated A1 demonstrated the highest decolorization rate (47.2%) when applied to a real wastewater sample after 96 h with a consequent reduction in COD from 592 to 494 ppm.

采用磁性氧化铁纳米粒子包被的高性能固定化菌株用于碱性蓝 41 偶氮染料（BB 41 染料）脱色。为了创建包被的细菌菌株，Raoultella Ornithinolytica sp。分离并鉴定为登录号 KT213695，然后涂有制造的磁性氧化铁纳米颗粒。使用SEM和SEM-EDX对包被细菌进行表征并验证其形态结构形成。包被的Raoultella Ornithinolyticasp。A1（涂层 A1）在起始浓度为 1600 ppm 时对 BB 41 染料产生 95.20% 的脱色，涂层 A1 的最佳剂量为 5 mL/L，pH 8，在 37 °C 静态条件下保持 24 小时。采用连续批次循环，每 24 小时添加 BB 41 染料（1600 ppm）四次，实现了 80.14% 的高脱色效率。此外，通过气相色谱-质谱分析 (GC-MS) 研究了 BB 41 染料生物降解的代谢物，结果显示对生物指示剂卤虫的毒性作用较小。此外，5 mL/L 的涂层 A1 在应用于真实废水样品 96 小时后显示出最高的脱色率 (47.2%)，COD 从 592 ppm 降低到 494 ppm。