检测 RNA-RNA 相互作用组,WIREs RNA

当前位置： X-MOL 学术 › WIREs RNA › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

检测 RNA-RNA 相互作用组
WIREs RNA ( IF 6.4 ) Pub Date : 2022-02-08 , DOI: 10.1002/wrna.1715
Suman Singh _{1,

2} , Sharmishtha Shyamal ₁ , Amaresh C. Panda ₁

Affiliation

在过去的十年中，各种类型的新型 RNA 分子及其在基因调控中的复杂性都出现了强劲增长。RNA 分子通过与其他生物分子（包括蛋白质、DNA 和 RNA）相互作用在细胞事件中发挥关键作用。已经确定 RNA-RNA 相互作用通过调节 RNA 分子的生物发生和功能在几个生物过程中发挥关键作用。有趣的是，RNA-RNA 相互作用通过剪接或反向剪接调节多种 RNA 分子的生物发生，包括 mRNA、microRNA、tRNA 和 circRNA。rRNA、tRNA 和 snRNA 等结构化 RNA 通过分子内 RNA-RNA 相互作用实现其功能构象。此外，许多分子间 RNA-RNA 相互作用的功能后果已在基因表达调控中得到广泛研究。因此，了解真核生物中 RNA-RNA 相互作用的机制和功能至关重要。几十年来，传统上，RNA-RNA 相互作用已通过多种生化方法进行鉴定。高通量 RNA 测序技术的出现彻底改变了细胞中全球 RNA-RNA 相互作用组的鉴定及其在 RNA 结构和基因表达调控中的重要性。尽管这些技术揭示了数以万计的分子内和分子间 RNA-RNA 相互作用，但我们进一步期待未来用于检测转录组范围的 RNA-RNA 相互作用的无偏和定量高通量技术。具有检测 RNA-RNA 相互作用组的能力，

"点击查看英文标题和摘要"

Detecting RNA–RNA interactome

The last decade has seen a robust increase in various types of novel RNA molecules and their complexity in gene regulation. RNA molecules play a critical role in cellular events by interacting with other biomolecules, including protein, DNA, and RNA. It has been established that RNA–RNA interactions play a critical role in several biological processes by regulating the biogenesis and function of RNA molecules. Interestingly, RNA–RNA interactions regulate the biogenesis of diverse RNA molecules, including mRNAs, microRNAs, tRNAs, and circRNAs, through splicing or backsplicing. Structured RNAs like rRNA, tRNA, and snRNAs achieve their functional conformation by intramolecular RNA–RNA interactions. In addition, functional consequences of many intermolecular RNA–RNA interactions have been extensively studied in the regulation of gene expression. Hence, it is essential to understand the mechanism and functions of RNA–RNA interactions in eukaryotes. Conventionally, RNA–RNA interactions have been identified through diverse biochemical methods for decades. The advent of high-throughput RNA-sequencing technologies has revolutionized the identification of global RNA–RNA interactome in cells and their importance in RNA structure and function in gene expression regulation. Although these technologies revealed tens of thousands of intramolecular and intermolecular RNA–RNA interactions, we further look forward to future unbiased and quantitative high-throughput technologies for detecting transcriptome-wide RNA–RNA interactions. With the ability to detect RNA–RNA interactome, we expect that future studies will reveal the higher-order structures of RNA molecules and multi-RNA hybrids impacting human health and diseases.

更新日期：2022-02-08

点击分享查看原文

点击收藏

阅读更多本刊新发论文