Natural Products and Bioprospecting ( IF 4.8 ) Pub Date : 2022-07-22 , DOI: 10.1007/s13659-022-00349-w Sitian Zhang 1 , Shuyuan Mo 1 , Fengli Li 1 , Yaxin Zhang 1 , Jianping Wang 1 , Zhengxi Hu 1 , Yonghui Zhang 1
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Soil-derived fungi represent an insufficiently tapped reservoir for discovering new and bioactive natural products (NPs), and despite an ever-increasing number of unknown NPs have been discovered over the past few decades, much of the hidden biosynthetic potential is still in an urgent need to be disclosed. In this research, a chemical investigation was performed on a wetland soil-derived fungus Aspergillus calidoustus TJ403-EL05, leading to the isolation of a total of fourteen drimane sesquiterpenoids (1–14), incorporating three new ones, namely ustusols F–H (1–3). Their structures, comprising absolute configurations, were completely authenticated by widespread spectroscopic data, quantum chemical 13C NMR and ECD calculations, and X-ray crystallography experiments. Compound 14 exhibited moderate anti-inflammatory activity by inhibiting the LPS-induced NO release (IC50 = 25.6 μM).
Graphical Abstract
中文翻译：
来自湿地土壤衍生真菌 Aspergillus calidoustus TJ403-EL05 的 Drimane 倍半萜
土壤衍生真菌是发现新的和具有生物活性的天然产物 (NPs) 的一个未充分开发的水库，尽管在过去几十年中发现了越来越多的未知 NPs，但许多隐藏的生物合成潜力仍处于紧迫状态需要披露。在这项研究中，对湿地土壤衍生真菌Aspergillus calidoustus TJ403-EL05进行了化学调查，共分离出 14 种 drimane 倍半萜类化合物 ( 1 - 14 )，其中包含三种新的化合物，即 ustusols F-H ( 1 - 3）。它们的结构（包括绝对构型）已通过广泛的光谱数据、量子化学13 C NMR 和 ECD 计算以及 X 射线晶体学实验得到完全验证。化合物14通过抑制 LPS 诱导的 NO 释放表现出中等的抗炎活性 (IC 50 = 25.6 μM)。
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