A Systematic Study of Size Correlation and Young's Modulus Sensitivity for Cellular Mechanical Phenotyping by Microfluidic Approaches,Advanced Healthcare Materials

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A Systematic Study of Size Correlation and Young's Modulus Sensitivity for Cellular Mechanical Phenotyping by Microfluidic Approaches
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2022-07-19 , DOI: 10.1002/adhm.202200628
Minhui Liang ₁ , Jianwei Zhong ₁ , Ye Ai ₁

Affiliation

Cellular mechanical properties are a class of intrinsic biophysical markers for cell state and health. Microfluidic mechanical phenotyping methods have emerged as promising tools to overcome the challenges of low throughput and high demand for manual skills in conventional approaches. In this work, two types of microfluidic cellular mechanical phenotyping methods, contactless hydro-stretching deformability cytometry (lh-DC) and contact constriction deformability cytometry (cc-DC) are comprehensively studied and compared. Polymerized hydrogel beads with defined sizes are used to characterize a strong negative correlation between size and deformability in cc-DC (r = −0.95), while lh-DC presents a weak positive correlation (r = 0.13). Young's modulus sensitivity in cc-DC is size-dependent while it is a constant in lh-DC. Moreover, the deformability assessment for human breast cell line mixture suggests the lh-DC exhibits better differentiation capability of cells with different size distributions, while cc-DC provides higher sensitivity to identify cellular mechanical changes within a single cell line. This work is the first to present a quantitative study and comparison of size correlation and Young's modulus sensitivity of contactless and contact microfluidic mechanical phenotyping methods, which provides guidance to choose the most suitable cellular mechanical phenotyping platform for specific cell analysis applications.

中文翻译：

微流体方法对细胞机械表型的尺寸相关性和杨氏模量敏感性的系统研究

细胞力学特性是细胞状态和健康的一类内在生物物理标记。微流体机械表型分析方法已成为克服传统方法中低通量和对手工技能要求高的挑战的有前途的工具。在这项工作中，全面研究和比较了两种类型的微流体细胞机械表型分析方法，即非接触式水力拉伸变形能力细胞术 (lh-DC) 和接触收缩变形能力细胞术 (cc-DC)。具有确定尺寸的聚合水凝胶珠用于表征 cc-DC 中尺寸和可变形性之间的强负相关（r = -0.95），而 lh-DC 呈现弱正相关（r = 0.13）。cc-DC 中的杨氏模量敏感性与尺寸有关，而它在 lh-DC 中是常数。此外，对人乳腺细胞系混合物的可变形性评估表明，lh-DC 对具有不同大小分布的细胞表现出更好的分化能力，而 cc-DC 提供更高的灵敏度来识别单个细胞系内的细胞机械变化。这项工作首次对非接触式和接触式微流体机械表型方法的尺寸相关性和杨氏模量敏感性进行了定量研究和比较，为特定细胞分析应用选择最合适的细胞机械表型平台提供了指导。

更新日期：2022-07-19

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