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Determination of 5-Formyluracil via Oxime-Based Nucleotide-Metal Coordination
ChemBioChem ( IF 2.6 ) Pub Date : 2022-07-18 , DOI: 10.1002/cbic.202200355
Kaiyuan Zhang 1 , Boshi Fu 2 , Guangrong Zou 1 , Wei Yang 1 , Shen Yan 1 , Tian Tian 1 , Xiang Zhou 1
Affiliation  

After its reaction with 5-formyluracil, a small chemical molecule, 2-(aminooxy)-N-(quinolin-8-yl)acetamide, was used to regulate activities of DNA polymerase. Then, the content of 5-formyluracil sites in DNA samples was detected by isothermal amplification strategy. According to the calculation of experimental data, 14.7 5-formyluracil components per 106 nucleotides per Gy were obtained in the radiation process.

中文翻译:

肟基核苷酸-金属配位法测定 5-甲酰尿嘧啶

与5-甲酰尿嘧啶反应后,一种化学小分子2-(氨基氧基) -N- (喹啉-8-基)乙酰胺被用来调节DNA聚合酶的活性。然后,通过等温扩增策略检测DNA样品中5-甲酰尿嘧啶位点的含量。根据实验数据计算,辐射过程中每10 6个核苷酸/Gy 得到14.7 个5-甲酰尿嘧啶组分。
更新日期:2022-07-18
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