基于 TMB-AgNPs@COF 的 SERS 探针快速检测饮料中的葡萄糖,Vibrational Spectroscopy

当前位置： X-MOL 学术 › Vib. Spectrosc. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

基于 TMB-AgNPs@COF 的 SERS 探针快速检测饮料中的葡萄糖
Vibrational Spectroscopy ( IF 2.7 ) Pub Date : 2022-07-19 , DOI: 10.1016/j.vibspec.2022.103411
Jialu Shen , Chun Zhu , Lei Li , Taiqun Yang , Yamin Wu , Chaoqun Ma , Jiao Gu , Hui Gao , Zichen Yang , Zirui Wang , Xiaoqian Qiu , Lvyuan Zhong , Anqi Hu , Anlan Huang , Jinzeng Xu , Senqi Guo , Wenzhi Yin , Guoqing Chen

饮料（尤其是无糖饮料）中葡萄糖的检测具有重要的现实意义。传统的葡萄糖检测方法是利用葡萄糖氧化酶进行酶促反应，耗时较长。在此，基于 TMB-AgNPs@COF 的 SERS 探针被设计用于快速检测饮料中的葡萄糖。SERS探针以银纳米粒子负载共价有机骨架（COFs）为底物，引入3,3',5,5'-四甲基联苯胺（TMB）作为信号分子，解决了葡萄糖SERS信号弱的问题。制造的 SERS 探针表示为 TMB-AgNPs@COF。检测机制基于 TMB 和葡萄糖与 AgNPs@COF 底物的竞争性结合。高斯理论模拟验证了葡萄糖更容易与底物结合。加入葡萄糖后，TMB 的 SERS 信号逐渐减弱。SERS光谱也可以证明底物和葡萄糖之间的相互作用。通过应用该方法，我们可以实现饮料中0.096 μg/mL的检测限。饮料回收实验中的相对标准偏差为 2.63 %- 4.89 %。整个检测时间小于5 min，底物重现性好。因此，该方法被证实具有检测饮料中葡萄糖的高潜力。89% 在饮料的回收实验中。整个检测时间小于5 min，底物重现性好。因此，该方法被证实具有检测饮料中葡萄糖的高潜力。89% 在饮料的回收实验中。整个检测时间小于5 min，底物重现性好。因此，该方法被证实具有检测饮料中葡萄糖的高潜力。

"点击查看英文标题和摘要"

TMB-AgNPs@COF based SERS probe for the rapid detection of glucose in drinks

The detection of glucose in drinks (especially for sugar-free drinks) is of great practical significance. The traditional method for the detection of glucose was based on an enzymatic reaction using glucose oxidase, which takes a long time. Herein, a TMB-AgNPs@COF based SERS probe was designed for the rapid detection of glucose in drinks. The SERS probe was fabricated using silver nanoparticles loaded covalent organic frameworks (COFs) as substrate, 3,3',5,5'-Tetramethylbenzidine (TMB) was introduced as a signal molecule to solve the problem of weak SERS signal of glucose. The fabricated SERS probe is denoted as TMB-AgNPs@COF. The detection mechanism was based on the competitive binding of TMB and glucose with AgNPs@COF substrate. Glucose was more likely to bond with the substrate which was verified by Gaussian theory simulation. The SERS signal of TMB gradually decreased when glucose was introduced. The interaction between the substrate and glucose can also be demonstrated by SERS spectroscopy. By applying this method, we could achieve the detection limit of 0.096 μg/mL in drinks. The relative standard deviations were 2.63 %− 4.89 % in the recovery experiments of drinks. The whole detection time was less than 5 min and the substrate had good reproducibility. Therefore, the method was confirmed with a high potential for the detection of glucose in drinks.

更新日期：2022-07-19

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南