包裹人脐带间充质干细胞的混合水凝胶可促进糖尿病伤口愈合,Journal of Materials Science: Materials in Medicine

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包裹人脐带间充质干细胞的混合水凝胶可促进糖尿病伤口愈合
Journal of Materials Science: Materials in Medicine ( IF 4.2 ) Pub Date : 2022-07-18 , DOI: 10.1007/s10856-022-06681-4
Hongjie Xu _{1,

2} , Jingjing Wang ₃ , Di Wu ₂ , Dajiang Qin _{1,

2}

Affiliation

背景

糖尿病伤口是糖尿病的严重并发症。干细胞被认为是治疗糖尿病皮肤伤口的一种有前途的疗法。水凝胶可以为细胞粘附和存活提供生态位，以提高干细胞治疗的疗效，但开发具有合适性能的水凝胶仍然是一个巨大的挑战。因此，我们的研究旨在将优化的水凝胶与干细胞相结合，以改善复杂的糖尿病伤口治疗。

方法

本研究从甲基丙烯酸明胶 (GelMA) 和壳聚糖-儿茶酚 (Chi-C) 构建了一种具有低毒性和可调节机械性能的水凝胶，并封装了人脐带间充质干细胞 (hUMSCs) 来修复全层糖尿病伤口。

结果

我们探索了机械刚度与细胞增殖和分化能力之间的关系，发现具有最佳刚度的 10% GelMA 水凝胶可改善体外 hUMSCs 的粘附、增殖和分化能力维持。辅以优化的水凝胶包封hUMSCs，大大加快了糖尿病伤口的愈合过程，包括加速伤口闭合，抑制炎症因子TNF-α和IL-1β的分泌，促进血管再生和hUMSCs治疗后的胶原蛋白沉积。

结论

优化的包裹hUMSCs的水凝胶改善了糖尿病伤口的愈合，对糖尿病并发症的治疗具有广泛的意义。

"点击查看英文标题和摘要"

A hybrid hydrogel encapsulating human umbilical cord mesenchymal stem cells enhances diabetic wound healing

Background

Diabetic wound is a severe complication of diabetes. Stem cell is considered as a promising therapy for diabetic skin wounds. Hydrogel can supply niche for cells adhesion and survival to improve the efficacy of stem cell therapy, but the development of hydrogel with suitable properties remains a great challenge. Thus, our study was conducted to combine an optimized hydrogel with stem cell to improve complex diabetic wound treatment.

Methods

This study constructed a hydrogel with low toxicity and adjustable mechanical properties from gelatin methacrylate (GelMA) and chitosan-catechol (Chi-C), and encapsulated human umbilical cord-mesenchymal stem cells (hUMSCs) to repair full-thickness diabetic wound.

Results

We explored the relationship between mechanical stiffness and cell proliferation and differentiation potency, and found 10% GelMA hydrogel with an optimal stiffness improved hUMSCs adhesion, proliferation, and differentiation potency maintenance in vitro. Assistant with optimized hydrogel encapsulating hUMSCs, diabetic wound healing process was greatly accelerated, including accelerated wound closure, inhibited secretion of inflammatory factors TNF-α and IL-1β, promoted vascular regeneration and collagen deposition after treatment of hUMSCs.