Cleavable Linker Incorporation into a Synthetic Dye-Nanobody-Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy,ChemBioChem

当前位置： X-MOL 学术 › ChemBioChem › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Cleavable Linker Incorporation into a Synthetic Dye-Nanobody-Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy
ChemBioChem ( IF 2.6 ) Pub Date : 2022-07-15 , DOI: 10.1002/cbic.202200395
Ayse Aktalay ₁ , Flavien Ponsot ₂ , Mariano L Bossi ₁ , Vladimir N Belov ₂ , Stefan W Hell _{1,

2}

Affiliation

Conjugate and cleave? No paradox: compare! The target protein fused with a FRET donor was labeled with a nanobody bearing a FRET acceptor (synthetic dye). Then the acceptor was cleaved off, and the fluorescence outputs before and after cleavage were compared. Here are the news from FRET, FLIM and STED microscopy involving an anti-GFP nanobody bound with a fluorophore via a disulfide bond.

中文翻译：

将可切割接头掺入合成染料-纳米抗体-荧光蛋白组装中：FRET、FLIM 和 STED 显微镜

共轭和裂解？没有悖论：比较！与 FRET 供体融合的靶蛋白被带有 FRET 受体（合成染料）的纳米抗体标记。然后将受体裂解掉，比较裂解前后的荧光输出。以下是来自 FRET、FLIM 和 STED 显微镜的新闻，涉及通过二硫键与荧光团结合的抗 GFP 纳米抗体。

更新日期：2022-07-15

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南