Transgalactosylation reaction is the penultimate step in the production of galactooligosaccharides (GOSs) which has prominent applications in the treatment of disorders. In the present study, partially purified β-galactosidase from Enterobacter aerogenes KCTC2190 was used for the synthesis of prebiotic GOSs. GOSs were produced using lactose as substrate. Structural elucidation of collected fractions of GOSs by liquid chromatography electrospray ionization mass spectrometry exhibited the appearance of major peaks of produced GOSs at m/z 241.20, 481.39, 365.11, 527.17, and 701.51 respectively. GOSs facilitated the growth of potential probiotic strains (Lactobacillus delbrueckii ssp. helveticus, Bifidobacterium bifidum, and Lactiplantibacillus plantarum) and liberated propionate and butyrate as principal short-chain fatty acids which established its prebiotic potency. Synbiotic combinations exhibited good antioxidant activities. Synbiotic combinations also exhibited antimicrobial activities against pathogenic microorganisms namely Staphylococcus aureus and Escherichia coli. Synbiotic combinations of GOSs and the respective probiotic microorganisms were able to decrease viable human bone cancer cells (MG-63).

转半乳糖基化反应是生产低聚半乳糖 (GOS) 的倒数第二个步骤，低聚半乳糖在疾病治疗中具有突出的应用。在本研究中，部分纯化的产气肠杆菌KCTC2190 β-半乳糖苷酶用于合成益生元 GOS。使用乳糖作为底物生产 GOS。通过液相色谱电喷雾电离质谱法对收集到的 GOS 馏分进行的结构解析表明，所产生的 GOS 的主峰分别位于m/z 241.20、481.39、365.11、527.17 和 701.51。GOS 促进了潜在益生菌菌株的生长（德氏乳杆菌瑞士亚种、双歧双歧杆菌, 和Lactiplantibacillus plantarum ) 并释放出丙酸和丁酸作为主要的短链脂肪酸，从而确立了其益生元效力。合生元组合表现出良好的抗氧化活性。合生元组合还表现出对病原微生物即金黄色葡萄球菌和大肠杆菌的抗菌活性。GOS 和相应益生菌微生物的合生元组合能够减少活的人骨癌细胞 (MG-63)。