Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas, such as semiconductor fabrication, biomedical imaging, and nanoscale material identification. Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view; however, those scanning microscopes still rely upon fluorescence labeling and complex optical systems. To overcome these limitations, we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm (alpha-blending process). This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location. Therefore, the number of images required to capture the entire field of view (200 × 200 μm) by using our acoustofluidic scanning nanoscope is reduced by 55-fold compared with previous designs. Moreover, the image quality is also greatly improved by applying an alpha-blending imaging technique, which is critical for accurately depicting and identifying nanoscale objects or processes. This dual-camera acoustofluidic nanoscope paves the way for enhanced nanoimaging with high resolution and a large field of view.

具有大视场的纳米级光学分辨率是许多研究和工业领域的关键特征，例如半导体制造、生物医学成像和纳米级材料识别。已经开发了几种扫描显微镜来解决分辨率和视场之间的反比关系；然而，这些扫描显微镜仍然依赖于荧光标记和复杂的光学系统。为了克服这些限制，我们开发了一种双摄像头声流纳米显微镜，具有无缝图像合并算法（阿尔法混合过程）。这种设计使我们能够同时对样品和微球进行精确成像，并准确跟踪颗粒路径和位置。因此，使用我们的声流控扫描纳米显微镜捕获整个视场（200×200μm）所需的图像数量比以前的设计减少了55倍。此外，通过应用阿尔法混合成像技术也大大提高了图像质量，这对于准确描绘和识别纳米级物体或过程至关重要。这种双摄像头声流控纳米显微镜为高分辨率和大视场的增强纳米成像铺平了道路。