Seabuckthorn (Hippophae salicifolia D. Don) is an economically important dioecious and deciduous actinorhizal tree species. Considering the contemporary interest in seabuckthorn as a future multipurpose crop, efforts have been initiated towards an assessment of genetic diversity prevailing in the natural populations inhabiting diverse geographical areas. In the present study, morphological and molecular diversity was studied in Hippophae salicifolia D. Don collections representing diverse ecological sites in the different geographical areas of the Garhwal and Kumaon regions of Uttarakhand, India. Morphological diversity was assessed by studying twenty-seven morphological characters. For standard identification of the color of leaves and berries, a color-coded reference (RHS)-based study was performed. By employing fifteen microsatellite markers, 109 alleles were detected and 71.5% of markers were polymorphic. Furthermore, polymorphic information content (PIC) (0.387), expected heterozygosity (H_e)/Nei’s Diversity (0.641), Wright’s fixation index (F_IS) (0.630), and Shannon’s informative index (I) (0.646) were calculated for the four populations simultaneously to study the phylogenetic relationships. The UPGMA-based dendrogram were also prepared by using morphological and molecular data. The dendrogram showed a demarcation between collections from different regions, although a few collections were found clustered with collections from the other regions. Assessment of genetic diversity was efficient and more informative using microsatellite markers. The findings of this study will be useful in future breeding and conservation programs in Hippophae salicifolia and related species.

沙棘（Hippophae salicifolia D. Don）是一种经济上重要的雌雄异株落叶放线菌树种。考虑到当代对沙棘作为未来多用途作物的兴趣，已经开始努力评估居住在不同地理区域的自然种群中普遍存在的遗传多样性。在本研究中，研究了沙棘的形态和分子多样性。D. 代表印度北阿坎德邦 Garhwal 和 Kumaon 地区不同地理区域不同生态遗址的 Don 收藏。通过研究 27 个形态特征来评估形态多样性。对于叶子和浆果颜色的标准识别，进行了基于颜色编码参考 (RHS) 的研究。通过使用 15 个微卫星标记，检测到 109 个等位基因，71.5% 的标记是多态性的。此外，多态信息含量 (PIC) (0.387)、预期杂合性 ( H _e )/Nei 的多样性 (0.641)、赖特固定指数 ( F _IS ) (0.630) 和香农信息指数 ( I) (0.646) 同时计算四个种群以研究系统发育关系。还通过使用形态学和分子数据制备了基于 UPGMA 的树状图。树状图显示了来自不同地区的馆藏之间的界限，尽管发现一些馆藏与来自其他地区的馆藏聚集在一起。使用微卫星标记对遗传多样性进行评估是有效的并且信息量更大。这项研究的结果将有助于未来的沙棘及相关物种的育种和保护计划。