A Novel Electrochemical Method for the Sensitive Determination of Aflatoxin B1 Using a Bivalent Binding Aptamer-cDNA Structure,Electroanalysis

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A Novel Electrochemical Method for the Sensitive Determination of Aflatoxin B1 Using a Bivalent Binding Aptamer-cDNA Structure
Electroanalysis ( IF 2.7 ) Pub Date : 2022-07-10 , DOI: 10.1002/elan.202200243
Elham Sameiyan ₁ , Parirokh Lavaee ₂ , Mohammad Ramezani ₂ , Mona Alibolandi ₁ , Zahra Khoshbin ₁ , Khalil Abnous ₃ , Seyed Mohammad Taghdisi ₁

Affiliation

In this study, a simple electrochemical sensing platform with the employment of a bivalent binding aptamer-cDNA probe (BBA-cDNA) structure is constructed for the detection of aflatoxin B₁ (AFB₁) as a mycotoxin. The BBA-cDNA structure is composed of two strands of aptamer (Apts) and their complementary strand (CS). Using a simple but accurate design, the presented measurement approach showed enhanced sensitivity and selectivity for AFB₁ detection with a LOD of 0.1 ng/mL. The approach presented in this study can be applied to the development of biosensors for the measurement of various toxins by substituting the proper aptamers and complementary strands.

中文翻译：

一种使用二价结合适体-cDNA 结构灵敏测定黄曲霉毒素 B1 的新型电化学方法

在这项研究中，构建了一个简单的电化学传感平台，该平台采用二价结合适体-cDNA 探针 (BBA-cDNA) 结构，用于检测作为霉菌毒素的黄曲霉毒素 B 1 ( _AFB 1 ₎。BBA-cDNA 结构由两条适体链 (Apts) 及其互补链 (CS) 组成。使用简单但准确的设计，所提出的测量方法显示出增强的 AFB ₁检测灵敏度和选择性，LOD 为 0.1 ng/mL。本研究中提出的方法可用于开发生物传感器，通过替换适当的适体和互补链来测量各种毒素。

更新日期：2022-07-10

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