Dynamin 在内吞位点引发超快内吞作用,Neuron

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Dynamin 在内吞位点引发超快内吞作用
Neuron ( IF 14.7 ) Pub Date : 2022-07-08 , DOI: 10.1016/j.neuron.2022.06.010
Yuuta Imoto ₁ , Sumana Raychaudhuri ₁ , Ye Ma ₂ , Pascal Fenske ₃ , Eduardo Sandoval ₁ , Kie Itoh ₁ , Eva-Maria Blumrich ₄ , Hideaki T Matsubayashi ₅ , Lauren Mamer ₃ , Fereshteh Zarebidaki ₃ , Berit Söhl-Kielczynski ₃ , Thorsten Trimbuch ₃ , Shraddha Nayak ₆ , Janet H Iwasa ₆ , Jian Liu ₇ , Bin Wu ₈ , Taekjip Ha ₉ , Takanari Inoue ₅ , Erik M Jorgensen ₁₀ , Michael A Cousin ₄ , Christian Rosenmund ₃ , Shigeki Watanabe ₁₁

Affiliation

Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA.
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Institute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; The Muir Maxwell Epilepsy Centre, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; Simons Initiatives for the Developing Brain, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK.
Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA.
Department of Biochemistry, University of Utah, Salt Lake City, UT 84112-0840, USA.
Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK.
The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA.
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA; Howard Hughes Medical Institute, Baltimore, MD 21205, USA.
HHMI, Department of Biology, University of Utah, Salt Lake City, UT 84112-0840, USA.
Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA.

动力蛋白在内吞作用期间介导囊泡从质膜分裂。通常，动力从胞质溶胶募集到内吞位点，需要数秒至数十秒。然而，在突触小泡回收过程中，神经元的超快内吞作用可将小泡内在化，速度最快可达 50 毫秒。在这里，我们证明 Dynamin 1 被预先招募到内吞位点以进行超快内吞作用。具体而言，Dynamin 1xA（Dynamin 1 的剪接变体）与 Syndapin 1 相互作用，在质膜上形成分子缩合物。Dynamin 1xA 分子的单粒子追踪证实了体内凝聚物的类液体特性。当 Dynamin 1xA 发生突变以破坏其与 Syndapin 1 的相互作用时，不会形成缩合物，因此超快内吞作用减慢 100 倍。从机制上讲，Syndapin 1 通过结合质膜充当接头，并将 Dynamin 1xA 储存在内吞位点。该缓存绕过了招募步骤并加速了突触的内吞作用。

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更新日期：2022-07-08

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